Optimization of fatty acid determination in selected fish and microalgal oils

Armenta, Roberto E., Scott, Spencer D., Burja, Adam M., Radianingtyas, Helia and Barrow, Colin J. 2009, Optimization of fatty acid determination in selected fish and microalgal oils, Chomatographia, vol. 70, no. 3-4, pp. 629-636, doi: 10.1365/s10337-009-1143-7.

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Title Optimization of fatty acid determination in selected fish and microalgal oils
Author(s) Armenta, Roberto E.
Scott, Spencer D.
Burja, Adam M.
Radianingtyas, Helia
Barrow, Colin J.ORCID iD for Barrow, Colin J. orcid.org/0000-0002-2153-7267
Journal name Chomatographia
Volume number 70
Issue number 3-4
Start page 629
End page 636
Total pages 8
Publisher Vieweg Verlag
Place of publication Berlin, Germany
Publication date 2009-08
ISSN 0009-5893
Keyword(s) Gas-liquid chromatography
Fatty acid methyl esters
Polyunsaturated fatty acids
Omega-3 fatty acids
Microalgal oil
Fish oil
Summary The use of ten fatty acid methyl ester reference standards coupled with a detailed quantification method was shown to significantly optimize the fatty acid determination of selected fish and microalgal oils when compared to methods that use only one reference standard (C19:0 or C23:0) as a relative response factor. When using the mixture of ten reference standards after transesterifying oils with NaOH/BF3, determination of total fatty acids, eicosapentaenoic acid and docosahexaenoic acid improved by an average of 7.3, 11.5 and 8.4%, respectively. Furthermore, improvements of 13.9, 18.9 and 6.8% of total fatty acids, EPA and DHA, respectively, were obtained when using the mixture of reference standards for fatty acid determination after directly extracting and transesterifying oil contained in microalgal cells with a mixture of methanol, HCl and chloroform. Fatty acid methyl ester standards dissolved in isooctane showed <5% variability throughout 130 days of stability testing when stored at −20 °C. The optimized method can be used for improving the quantification of fatty acids in both oils (fish and microalgal oils) and dry microalgal cells.
Language eng
DOI 10.1365/s10337-009-1143-7
Field of Research 100303 Fermentation
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2009, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019669

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