IgE cross-reactivity between the major peanut allergen Ara h 2 and tree nut allergens

de Leon, M.P., Drew, A.C., Glaspole, I.N., Suphioglu, C., O'Hehir, R.E. and Rolland, J.M. 2007, IgE cross-reactivity between the major peanut allergen Ara h 2 and tree nut allergens, Molecular immunology, vol. 44, no. 4, pp. 463-471, doi: 10.1016/j.molimm.2006.02.016.

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Title IgE cross-reactivity between the major peanut allergen Ara h 2 and tree nut allergens
Author(s) de Leon, M.P.
Drew, A.C.
Glaspole, I.N.
Suphioglu, C.ORCID iD for Suphioglu, C. orcid.org/0000-0003-0101-0668
O'Hehir, R.E.
Rolland, J.M.
Journal name Molecular immunology
Volume number 44
Issue number 4
Start page 463
End page 471
Publisher Pergamon
Place of publication Oxford, England
Publication date 2007-01
ISSN 0161-5890
Keyword(s) allergy
tree nuts
IgE cross-reactivity
Ara h 2
Summary Allergy to peanut and tree nuts is characterised by a high frequency of life-threatening anaphylactic reactions and typically lifelong persistence. Although peanut is the most common cause of nut allergy, peanut allergic patients are frequently also sensitive to tree nuts. It is not known if this is due to cross-reactivity between peanut and tree nut allergens. In this study, the major peanut allergen Ara h 2 was cloned from peanut cDNA, expressed in E. coli cells as a His-tag fusion protein and purified using a Ni-NTA column. Immunoblotting, ELISA and basophil activation indicated by CD63 expression all confirmed the IgE reactivity and biological activity of rAra h 2. To determine whether or not this allergen plays a role in IgE cross-reactivity between peanut and tree nuts, inhibition ELISA was performed. Pre-incubation of serum from peanut allergic patients with increasing concentrations of almond or Brazil nut extract inhibited IgE binding to rAra h 2. Purified rAra h 2-specific serum IgE antibodies also bound to proteins present in almond and Brazil nut extracts by immunoblotting. This indicates that the major peanut allergen, Ara h 2, shares common IgE-binding epitopes with almond and Brazil nut allergens, which may contribute to the high incidence of tree nut sensitisation in peanut allergic individuals.
Language eng
DOI 10.1016/j.molimm.2006.02.016
Field of Research 110199 Medical Biochemistry and Metabolomics not elsewhere classified
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2006, Elsevier Ltd
Persistent URL http://hdl.handle.net/10536/DRO/DU:30019692

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