Adiponectin decreases pyruvate dehydrogenase kinase 4 gene expression in obese- and diabetic derived

McAinch, A.J. and Cameron-Smith, D. 2009, Adiponectin decreases pyruvate dehydrogenase kinase 4 gene expression in obese- and diabetic derived, Diabetes, obesity and metabolism, vol. 11, no. 7, pp. 721-728, doi: 10.1111/j.1463-1326.2009.01042.x.

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Title Adiponectin decreases pyruvate dehydrogenase kinase 4 gene expression in obese- and diabetic derived
Author(s) McAinch, A.J.
Cameron-Smith, D.
Journal name Diabetes, obesity and metabolism
Volume number 11
Issue number 7
Start page 721
End page 728
Publisher Wiley Blackwell Publishing
Place of publication Oxford, England
Publication date 2009-07
ISSN 1462-8902
Keyword(s) AMPK
fat metabolism
Summary Aim: To investigate the effects of globular adiponectin (gAd) on gene expression and whether these effects are mediated through 3',5'-cyclic monophosphate-activated protein kinase in skeletal muscle myotubes obtained from lean, obese and obese diabetic individuals.

Methods: Rectus abdominus muscle biopsies were obtained from surgical patients to establish primary skeletal muscle cell cultures. Three distinct primary cell culture groups were established (lean, obese and obese diabetic; n = 7 in each group). Once differentiated, these cultures were then exposed to gAd or 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR) for 6 h.

Results: Stimulation with gAd decreased pyruvate dehydrogenase kinase 4 (PDK4) gene expression in the obese and diabetic samples (p ≤ 0.05) and increased cytochrome c oxidase (COX) subunit 4 (COXIV) gene expression in the myotubes derived from lean individuals only (p < 0.05). AICAR treatment also decreased PDK4 gene expression in the obese- and diabetic-derived myotubes (p ≤ 0.05) and increased the gene expression of the mitochondrial gene, COXIII, in the lean-derived samples only (p < 0.05).

Conclusions: This study demonstrated distinct disparity between myotubes derived from lean compared with obese and obese diabetic individuals following gAd and AICAR treatment. Further understanding of the regulation of PDK4 in obese and diabetic skeletal muscle and its interaction with adiponectin signalling is required as this appears to be an important early molecular event in these disease states that may improve blood glucose control and metabolic flux.
Language eng
DOI 10.1111/j.1463-1326.2009.01042.x
Field of Research 110306 Endocrinology
Socio Economic Objective 920104 Diabetes
HERDC Research category C3 Non-refereed articles in a professional journal
HERDC collection year 2009
Copyright notice ©2009, Blackwell Publishing Ltd
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Document type: Journal Article
Collections: Faculty of Health
School of Exercise and Nutrition Sciences
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Created: Wed, 30 Sep 2009, 12:48:44 EST by Sally Morrigan

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