COPD results in a reduction in UCP3 long mRNA and UCP3 protein content in types I and IIa skeletal muscle fibers.

Russell, Aaron, Somm, Emmanuel, Debigare, Richard, Hartley, Oliver, Richard, Denis, Giacomo, Gastaldi, Melotti, Astrid, Michaud, Annie, Giacobino, Jean-Paul, Muzzin, Patrick, LeBlanc, Pierre and Francois, Maltais 2004, COPD results in a reduction in UCP3 long mRNA and UCP3 protein content in types I and IIa skeletal muscle fibers., Journal of cardiopulmonary rehabilitation, vol. 24, no. 5, September - October, pp. 332-339.

Attached Files
Name Description MIMEType Size Downloads

Title COPD results in a reduction in UCP3 long mRNA and UCP3 protein content in types I and IIa skeletal muscle fibers.
Author(s) Russell, AaronORCID iD for Russell, Aaron
Somm, Emmanuel
Debigare, Richard
Hartley, Oliver
Richard, Denis
Giacomo, Gastaldi
Melotti, Astrid
Michaud, Annie
Giacobino, Jean-Paul
Muzzin, Patrick
LeBlanc, Pierre
Francois, Maltais
Journal name Journal of cardiopulmonary rehabilitation
Volume number 24
Issue number 5
Season September - October
Start page 332
End page 339
Publisher Lippincott Williams & Wilkins
Place of publication Philadelphia, Pa.
Publication date 2004
ISSN 0883-9212
Keyword(s) uncoupling protein
skeletal muscle
oxidative phosphorylation
Summary Purpose: Findings recently have shown coupling protein-3 (UCP3) content to be decreased in the skeletal muscle of patients with chronic obstructive pulmonary disease (COPD). Uncoupling protein-3 mRNA exists as two isoforms: long (UCP3L) and short (UCP3S). The UCP3 protein is expressed the least in oxidative and the most in glycolytic muscle fibers. Levels of UCP3 have been associated positively with intramyocellular triglyceride (IMTG) contents in conditions of altered fatty acid metabolism. As a source for muscle free fatty acid metabolism, IMTG is decreased in COPD. The current study completely characterized all the parameters of UCP3 expression (ie, UCP3L and UCP3S mRNA expression in whole muscle samples) and UCP3 protein content as well as IMTG content in the different fiber types in patients with COPD and healthy control subjects.

Methods: Using real-time polymerase chain reaction, UCP3 gene expression was quantified. Skeletal muscle fiber type and UCP3 protein and IMTG content were measured using immunofluorescence and Oil red oil staining, respectively.

Results: The findings showed that UCP3L mRNA expression was 44% lower (P < .005) in the patients with COPD than in the control subjects, whereas the UCP3S mRNA content was similar in the two groups. As compared with control subjects, UCP3 protein content was decreased by 89% and 83% and the IMTG content by 64% and 54%, respectively, in types I and IIa fibers (P < .0167) of patients with COPD, whereas they were unchanged in IIx fibers.

Conclusions: The reduced UCP3 and IMTG content in the more oxidative fibers may be linked to the altered muscle fatty acid metabolism associated with COPD. Further studies are required to determine the exact role and clinical relevance of the reduced UCP3 content in patients with COPD.
Language eng
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Persistent URL

Connect to link resolver
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in TR Web of Science
Scopus Citation Count Cited 18 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 862 Abstract Views, 0 File Downloads  -  Detailed Statistics
Created: Tue, 19 Jan 2010, 13:52:22 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact