Identification and characterization of genes conferring salt tolerance to Escherichia coli from pond water metagenome Kapardar, Raj Kishor, Ranjan, Ravi, Grover, Amit, Puri, Munish and Sharma, Rakesh 2010, Identification and characterization of genes conferring salt tolerance to Escherichia coli from pond water metagenome, Bioresource technology, vol. 101, no. 11, pp. 3917-3924, doi: 10.1016/j.biortech.2010.01.017. Attached Files Name Description MIMEType Size Downloads Title Identification and characterization of genes conferring salt tolerance to Escherichia coli from pond water metagenome Formatted title Identification and characterization of genes conferring salt tolerance to Escherichia coli from pond water metagenome Author(s) Kapardar, Raj Kishor Ranjan, Ravi Grover, Amit Puri, Munish orcid.org/0000-0003-2469-3326 Sharma, Rakesh Journal name Bioresource technology Volume number 101 Issue number 11 Start page 3917 End page 3924 Total pages 8 Publisher Elsevier Place of publication Amsterdam, The Netherlands Publication date 2010-06 ISSN 0960-8524 1873-2976 Keyword(s) metagenome salt tolerance Enoyl-CoA hydratase general stress protein Summary Metagenomics provides culture-independent access to gene pool of the whole microbial communities. To identify genes responsible for salt tolerance in unculturable bacteria, Escherichia coli clones were enriched with an ability to grow at inhibitory NaCl concentrations (750 mM) from a pond water metagenomic library. From two unique clones, genes encoding for proteins with similarity to a putative general stress protein (GspM) harbouring GsiB domain and a putative enoyl-CoA hydratase (EchM) were identified to be responsible for salt tolerance. The gspM was expressed by its native promoter whereas the echM was expressed from the lacZ promoter of the plasmid. EchM was overexpressed with a hexahistidyl tag. Purified EchM showed crotonyl-CoA hydratase activity. These genes have potential application in generating salt tolerant recombinant bacteria or transgenic plants. Language eng DOI 10.1016/j.biortech.2010.01.017 Field of Research 100205 Environmental Marine Biotechnology HERDC Research category C1.1 Refereed article in a scholarly journal HERDC collection year 2010 Copyright notice ©2010, Elsevier Ltd. Persistent URL http://hdl.handle.net/10536/DRO/DU:30023627 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment Centre for Biotechnology and Interdisciplinary Sciences (BioDeakin) Institute for Technology Research and Innovation GTP Research Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Wed, 10 Feb 2010, 14:06:57 EST Mon, 21 Jun 2010, 14:58:36 EST Tue, 22 Jun 2010, 12:45:41 EST Tue, 22 Jun 2010, 14:38:25 EST Tue, 22 Jun 2010, 14:39:47 EST Wed, 23 Jun 2010, 08:56:22 EST Wed, 23 Jun 2010, 09:08:20 EST Mon, 28 Jun 2010, 16:25:24 EST Mon, 28 Jun 2010, 19:16:32 EST Wed, 15 Feb 2012, 11:41:26 EST Mon, 16 Jun 2014, 14:22:22 EST Thu, 11 Dec 2014, 16:26:43 EST Filtered Full Citation counts:   Cited 28 times in TR Web of Science Cited 39 times in Scopus Search Google Scholar Access Statistics: 803 Abstract Views, 2 File Downloads  -  Detailed Statistics Created: Wed, 10 Feb 2010, 14:06:52 EST by Munish Puri