Reverse ascertainment bias in microsatellite allelic diversity in owls (Aves, Strigiformes) Hogan, Fiona E, Cooke, Raylene. and Norman, Janette A 2009, Reverse ascertainment bias in microsatellite allelic diversity in owls (Aves, Strigiformes), Conservation genetics, vol. 10, no. 3, pp. 635-638, doi: 10.1007/s10592-008-9595-0. Attached Files Name Description MIMEType Size Downloads Title Reverse ascertainment bias in microsatellite allelic diversity in owls (Aves, Strigiformes) Author(s) Hogan, Fiona E Cooke, Raylene. orcid.org/0000-0002-8843-7113 Norman, Janette A Journal name Conservation genetics Volume number 10 Issue number 3 Start page 635 End page 638 Total pages 4 Publisher Springer Netherlands Place of publication Dordrecht , Netherlands Publication date 2009-06 ISSN 1566-0621 1572-9737 Keyword(s) cross-species amplification Strigidae Tytonidae microsatellite reverse ascertainment bias Summary A rich source of markers may be overlooked by screening for polymorphism in the source species only. We screened 129 microsatellite loci isolated from the powerful owl (Ninox strenua) against two closely related species; Ninox  connivens and Ninox novaeseelandiae. From the screening effort 20 polymorphic markers were isolated, including six loci which were originally discarded as they were monomorphic in the source species. Further cross-species amplification of all 20 loci across species from two families, Strigidae and Tytonidae, revealed unusually high levels of polymorphism within closely related species, and limited success within phylogenetically distant species. Routine screening of multiple  species during the marker development phase can yield a wider range of  polymorphic markers which can subsequently enhance cross-species  amplification attempts. Language eng DOI 10.1007/s10592-008-9595-0 Field of Research 060411 Population, Ecological and Evolutionary Genetics Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2008, Springer Science + Business Media B.V. Persistent URL http://hdl.handle.net/10536/DRO/DU:30024939 Document type: Journal Article Collections: Faculty of Arts and Education Faculty of Science, Engineering and Built Environment School of Life and Environmental Sciences Higher Education Research Group Centre for Integrative Ecology Related Links Link Description Connected to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Mon, 08 Mar 2010, 13:52:03 EST Mon, 08 Mar 2010, 13:53:59 EST Fri, 23 Apr 2010, 09:21:36 EST Tue, 27 Apr 2010, 12:33:09 EST Tue, 27 Apr 2010, 12:36:47 EST Mon, 16 Jun 2014, 14:47:56 EST Wed, 27 Aug 2014, 22:12:04 EST Filtered Full Citation counts:   Cited 8 times in TR Web of Science Cited 7 times in Scopus Search Google Scholar Access Statistics: 927 Abstract Views, 3 File Downloads  -  Detailed Statistics Created: Mon, 08 Mar 2010, 13:51:56 EST by Teresa Treffry