Sindbis virus vectors elicit hemagglutinin-specific humoral and cellular immune responses and offer a dose-sparing strategy for vaccination Miller, Annett, Center, Rob J., Stambas, John, Deliyannis, Georgia, Doherty, Peter C., Howard, Jane L., Turner, Stephen J. and Purcell, Damian F.J. 2008, Sindbis virus vectors elicit hemagglutinin-specific humoral and cellular immune responses and offer a dose-sparing strategy for vaccination, Vaccine, vol. 26, no. 44, pp. 5641-5648, doi: 10.1016/j.vaccine.2008.07.102. Attached Files Name Description MIMEType Size Downloads Title Sindbis virus vectors elicit hemagglutinin-specific humoral and cellular immune responses and offer a dose-sparing strategy for vaccination Author(s) Miller, Annett Center, Rob J. Stambas, John orcid.org/0000-0002-5690-2551 Deliyannis, Georgia Doherty, Peter C. Howard, Jane L. Turner, Stephen J. Purcell, Damian F.J. Journal name Vaccine Volume number 26 Issue number 44 Start page 5641 End page 5648 Total pages 8 Publisher Elsevier Ltd. Place of publication Guildford, England Publication date 2008-10-16 ISSN 0264-410X 1873-2518 Keyword(s) influenza vaccine sindbis Summary We report here on the use of a Sindbis virus-based DNA-launch RNA replicon vector (pSIN-HA) that expresses influenza hemagglutinin (HA) as an immunogen. Immunization of mice with pSIN-HA generated anti-HA antibody and CTL responses and resulted in lower lung viral titers after influenza challenge when compared to controls. Importantly, immunization with a low dose of pSIN-HA mediated significantly reduced lung viral titers following challenge at 43 weeks after the final immunization. In contrast, immunization with a non-replicon DNA vector expressing HA failed to mediate reduced lung viral titer at the same dose. This demonstrated the dose-sparing capacity of the SIN vector system and its ability to stimulate long-term memory responses, properties that are highly desirable in any vaccine formulation. Language eng DOI 10.1016/j.vaccine.2008.07.102 Field of Research 110804 Medical Virology Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences HERDC Research category C1.1 Refereed article in a scholarly journal Grant ID NHMRC 508902 Copyright notice ©Copyright 2008 Elsevier Ltd All rights reserved. Persistent URL http://hdl.handle.net/10536/DRO/DU:30028894 Document type: Journal Article Collections: Faculty of Health School of Medicine Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Wed, 26 May 2010, 16:07:02 EST Fri, 30 Jul 2010, 09:55:17 EST Fri, 30 Jul 2010, 09:56:54 EST Fri, 30 Jul 2010, 09:58:03 EST Fri, 30 Jul 2010, 09:58:57 EST Fri, 30 Jul 2010, 10:03:25 EST Fri, 30 Jul 2010, 10:04:25 EST Fri, 30 Jul 2010, 12:15:13 EST Wed, 17 Oct 2012, 13:54:51 EST Mon, 16 Jun 2014, 15:49:30 EST Thu, 11 Dec 2014, 16:38:06 EST Filtered Full Citation counts:   Cited 10 times in TR Web of Science Cited 10 times in Scopus Search Google Scholar Access Statistics: 929 Abstract Views, 2 File Downloads  -  Detailed Statistics Created: Wed, 26 May 2010, 16:07:02 EST