•  Home
  • Library
  • DRO home
Submit research Contact DRO

DRO

High-performance liquid chromatography with post-column 2,2′-diphenyl-1-picrylhydrazyl radical scavenging assay : methodological considerations and application to complex samples

McDermott, Geoffrey P., Noonan, Laura K., Mnatsakanyan, Mariam, Shalliker, R. Andrew, Conlan, Xavier A., Barnett, Neil W. and Francis, Paul S. 2010, High-performance liquid chromatography with post-column 2,2′-diphenyl-1-picrylhydrazyl radical scavenging assay : methodological considerations and application to complex samples, Analytica chimica acta, vol. 675, no. 1, pp. 76-82, doi: 10.1016/j.aca.2010.06.041.

Attached Files
Name Description MIMEType Size Downloads

Title High-performance liquid chromatography with post-column 2,2′-diphenyl-1-picrylhydrazyl radical scavenging assay : methodological considerations and application to complex samples
Author(s) McDermott, Geoffrey P.
Noonan, Laura K.
Mnatsakanyan, Mariam
Shalliker, R. Andrew
Conlan, Xavier A.ORCID iD for Conlan, Xavier A. orcid.org/0000-0003-0829-0551
Barnett, Neil W.
Francis, Paul S.ORCID iD for Francis, Paul S. orcid.org/0000-0003-4165-6922
Journal name Analytica chimica acta
Volume number 675
Issue number 1
Start page 76
End page 82
Total pages 7
Publisher Elsevier B.V.
Place of publication Amsterdam, The Netherlands
Publication date 2010-08-18
ISSN 0003-2670
1873-4324
Keyword(s) 2,2´-Diphenyl-1-picrylhydrazyl
radical scavenging assay
antioxidants
high-performance liquid chromatography
green tea
thyme
Summary An improved post-column 2,2´-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging assay for the screening of antioxidants in complex matrices was developed. Experimental parameters believed to be influential to DPPH• response were studied in a univariate approach. Optimum conditions were found to be: 5×10−5M DPPH• reagent prepared in a 75% methanol: 25% 40mM citric acid–sodium citrate buffer (pH 6) solution, degassed with nitrogen; reaction coil of 2m×0.25mm i.d. PEEK tubing; detection at 521 nm; analysis at room temperature. The analytical utility of this protocol was evaluated by screening for antioxidants in thyme and green tea, in comparison with two commonly employed methodologies.
Notes Available online 23 July 2010
Language eng
DOI 10.1016/j.aca.2010.06.041
Field of Research 030108 Separation Science
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2010
Related work DU:30042598
Copyright notice ©2010, Elsevier B.V.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30031280

Document type: Journal Article
Collections: Faculty of Science, Engineering and Built Environment
School of Life and Environmental Sciences
Centre for Biotechnology and Interdisciplinary Sciences (BioDeakin)
Related Links
Link Description
Connect to published version (restricted access)
Go to link with your DU access privileges
 
Connect to link resolver
 
Link to Related Work
  • Determination of antioxidants using chemiluminescence detection Thesis (deposited 22-02-2012)
  • Stable tris(2,2'-bipyridine)ruthenium(III) for chemiluminescence detection Journal Article (deposited 05-12-2011)
  • Direct detection of biologically significant thiols and disulfides with manganese(IV) chemiluminescence Journal Article (deposited 05-12-2011)
  • Determination of intracellular glutathione and glutathione disulfide using high performance liquid chromatography with acidic potassium permanganate chemiluminescence detection Journal Article (deposited 05-12-2011)
  • High performance liquid chromatography with two simultaneous on-line antioxidant assays : evaluation and comparison of espresso coffees Journal Article (deposited 23-11-2010)
  • High-performance liquid chromatography with post-column 2,2′-diphenyl-1-picrylhydrazyl radical scavenging assay : methodological considerations and application to complex samples Journal Article (deposited 22-11-2010)
  • The analysis of café espresso using two-dimensional reversed phase–reversed phase high performance liquid chromatography with UV-absorbance and chemiluminescence detection Journal Article (deposited 12-11-2010)
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 23 times in TR Web of Science
Scopus Citation Count Cited 24 times in Scopus Google Scholar Search Google Scholar
Access Statistics: 1044 Abstract Views, 4 File Downloads  -  Detailed Statistics
Created: Mon, 22 Nov 2010, 13:44:24 EST by Teresa Treffry

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.