High-performance liquid chromatography with post-column 2,2′-diphenyl-1-picrylhydrazyl radical scavenging assay : methodological considerations and application to complex samples McDermott, Geoffrey P., Noonan, Laura K., Mnatsakanyan, Mariam, Shalliker, R. Andrew, Conlan, Xavier A., Barnett, Neil W. and Francis, Paul S. 2010, High-performance liquid chromatography with post-column 2,2′-diphenyl-1-picrylhydrazyl radical scavenging assay : methodological considerations and application to complex samples, Analytica chimica acta, vol. 675, no. 1, pp. 76-82, doi: 10.1016/j.aca.2010.06.041. Attached Files Name Description MIMEType Size Downloads Title High-performance liquid chromatography with post-column 2,2′-diphenyl-1-picrylhydrazyl radical scavenging assay : methodological considerations and application to complex samples Author(s) McDermott, Geoffrey P. Noonan, Laura K. Mnatsakanyan, Mariam Shalliker, R. Andrew Conlan, Xavier A. orcid.org/0000-0003-0829-0551 Barnett, Neil W. Francis, Paul S. orcid.org/0000-0003-4165-6922 Journal name Analytica chimica acta Volume number 675 Issue number 1 Start page 76 End page 82 Total pages 7 Publisher Elsevier B.V. Place of publication Amsterdam, The Netherlands Publication date 2010-08-18 ISSN 0003-2670 1873-4324 Keyword(s) 2,2´-Diphenyl-1-picrylhydrazyl radical scavenging assay antioxidants high-performance liquid chromatography green tea thyme Summary An improved post-column 2,2´-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging assay for the screening of antioxidants in complex matrices was developed. Experimental parameters believed to be influential to DPPH• response were studied in a univariate approach. Optimum conditions were found to be: 5×10−5M DPPH• reagent prepared in a 75% methanol: 25% 40mM citric acid–sodium citrate buffer (pH 6) solution, degassed with nitrogen; reaction coil of 2m×0.25mm i.d. PEEK tubing; detection at 521 nm; analysis at room temperature. The analytical utility of this protocol was evaluated by screening for antioxidants in thyme and green tea, in comparison with two commonly employed methodologies. Notes Available online 23 July 2010 Language eng DOI 10.1016/j.aca.2010.06.041 Field of Research 030108 Separation Science Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences HERDC Research category C1 Refereed article in a scholarly journal HERDC collection year 2010 Related work DU:30042598 Copyright notice ©2010, Elsevier B.V. Persistent URL http://hdl.handle.net/10536/DRO/DU:30031280 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Life and Environmental Sciences Centre for Biotechnology and Interdisciplinary Sciences (BioDeakin) Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Link to Related Work Determination of antioxidants using chemiluminescence detection Thesis (deposited 22-02-2012) Stable tris(2,2'-bipyridine)ruthenium(III) for chemiluminescence detection Journal Article (deposited 05-12-2011) Direct detection of biologically significant thiols and disulfides with manganese(IV) chemiluminescence Journal Article (deposited 05-12-2011) Determination of intracellular glutathione and glutathione disulfide using high performance liquid chromatography with acidic potassium permanganate chemiluminescence detection Journal Article (deposited 05-12-2011) High performance liquid chromatography with two simultaneous on-line antioxidant assays : evaluation and comparison of espresso coffees Journal Article (deposited 23-11-2010) High-performance liquid chromatography with post-column 2,2′-diphenyl-1-picrylhydrazyl radical scavenging assay : methodological considerations and application to complex samples Journal Article (deposited 22-11-2010) The analysis of café espresso using two-dimensional reversed phase–reversed phase high performance liquid chromatography with UV-absorbance and chemiluminescence detection Journal Article (deposited 12-11-2010)   Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Mon, 22 Nov 2010, 13:44:25 EST Mon, 22 Nov 2010, 13:45:12 EST Wed, 24 Nov 2010, 07:39:55 EST Wed, 24 Nov 2010, 11:07:55 EST Wed, 24 Nov 2010, 11:09:19 EST Wed, 24 Nov 2010, 13:27:21 EST Tue, 30 Nov 2010, 13:11:47 EST Mon, 06 Dec 2010, 13:54:24 EST Mon, 06 Dec 2010, 14:29:34 EST Wed, 15 Jun 2011, 09:47:37 EST Wed, 15 Jun 2011, 09:53:02 EST Fri, 30 Mar 2012, 13:01:11 EST Thu, 03 Oct 2013, 10:47:31 EST Mon, 16 Jun 2014, 16:51:49 EST Fri, 22 Aug 2014, 13:52:24 EST Wed, 27 Aug 2014, 22:28:53 EST Filtered Full Citation counts:   Cited 23 times in TR Web of Science Cited 24 times in Scopus Search Google Scholar Access Statistics: 1044 Abstract Views, 4 File Downloads  -  Detailed Statistics Created: Mon, 22 Nov 2010, 13:44:24 EST by Teresa Treffry