Mechanisms of nitric oxide-mediated neurogenic,vasodilation in mesenteric resistance arteries of toad, Bufo marinus Jennings, Brett L. and Donald, John A. 2010, Mechanisms of nitric oxide-mediated neurogenic,vasodilation in mesenteric resistance arteries of toad, Bufo marinus, American journal of physiology regulatory intergrative and comparative physiology, vol. 298, no. 3, pp. R767-R775, doi: 10.1152/ajpregu.00148.2009. Attached Files Name Description MIMEType Size Downloads Title Mechanisms of nitric oxide-mediated neurogenic,vasodilation in mesenteric resistance arteries of toad, Bufo marinus Formatted title Mechanisms of nitric oxide-mediated neurogenic,vasodilation in mesenteric resistance arteries of toad, Bufo marinus Author(s) Jennings, Brett L. Donald, John A. orcid.org/0000-0001-5930-2642 Journal name American journal of physiology regulatory intergrative and comparative physiology Volume number 298 Issue number 3 Start page R767 End page R775 Total pages 9 Publisher American Physiological Society Place of publication Bethesda, Md. Publication date 2010-03-01 ISSN 0363-6119 1522-1490 Keyword(s) amphibian autonomic nervous system nitric oxide synthase endothelium Summary This study determined the role of nitric oxide (NO) in neurogenic vasodilation in mesenteric resistance arteries of the toad Bufo marinus. NO synthase (NOS) was anatomically demonstrated in perivascular nerves, but not in the endothelium. ACh and nicotine caused TTX-sensitive neurogenic vasodilation of mesenteric arteries. The ACh-induced vasodilation was endothelium-independent and was mediated by the NO/soluble guanylyl cyclase signaling pathway, inasmuch as the vasodilation was blocked by the soluble guanylyl cyclase inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one and the NOS inhibitors Nω- nitro-L-arginine methyl ester and Nω-nitro-L-arginine. Furthermore, the ACh-induced vasodilation was significantly decreased by the more selective neural NOS inhibitor N5-(1-imino-3-butenyl)-L-ornithine. The nicotine-induced vasodilation was endothelium-independent and mediated by NO and calcitonin gene-related peptide (CGRP), inasmuch as pretreatment of mesenteric arteries with a combination of Nω-nitro-L-arginine and the CGRP receptor antagonist CGRP-(8–37) blocked the vasodilation. Clotrimazole significantly decreased the ACh-induced response, providing evidence that a component of the NO vasodilation involved Ca2+-activated K+ or voltage-gated K+ channels. These data show that NO control of mesenteric resistance arteries of toad is provided by nitrergic nerves, rather than the endothelium, and implicate NO as a potentially important regulator of gut blood flow and peripheral blood pressure. Notes First published January 13, 2010 Language eng DOI 10.1152/ajpregu.00148.2009 Indigenous content off Field of Research 111603 Systems Physiology Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences HERDC Research category C1 Refereed article in a scholarly journal HERDC collection year 2010 Copyright notice ©2010, American Physiological Society Persistent URL http://hdl.handle.net/10536/DRO/DU:30032912 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Life and Environmental Sciences Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges   Connect to Elements publication management system Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Thu, 17 Feb 2011, 11:05:38 EST Thu, 17 Feb 2011, 11:06:40 EST Tue, 08 Mar 2011, 10:39:32 EST Tue, 08 Mar 2011, 15:29:10 EST Tue, 08 Mar 2011, 15:32:07 EST Fri, 18 Mar 2011, 13:26:19 EST Fri, 18 Mar 2011, 13:28:20 EST Fri, 18 Mar 2011, 13:36:25 EST Fri, 18 Mar 2011, 13:37:50 EST Tue, 14 Jun 2011, 13:28:37 EST Mon, 16 Jun 2014, 17:23:50 EST Tue, 04 Aug 2020, 14:40:08 EST Filtered Full Citation counts:   Cited 13 times in TR Web of Science Cited 13 times in Scopus Search Google Scholar Access Statistics: 907 Abstract Views, 5 File Downloads  -  Detailed Statistics Created: Thu, 17 Feb 2011, 11:05:37 EST by Teresa Treffry