Three-dimensional tissue scaffolds from interbonded poly(e-caprolactone) fibrous matrices with controlled porosity

Tang, Yanwei, Wong, Cynthia, Wang, Hongxia, Sutti, Alessandra, Kirkland, Mark, Wang, Xungai and Lin, Tong 2011, Three-dimensional tissue scaffolds from interbonded poly(e-caprolactone) fibrous matrices with controlled porosity, Tissue engineering. Part C. methods, vol. 17, no. 2, pp. 209-217, doi: 10.1089/ten.tec.2010.0223.

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Title Three-dimensional tissue scaffolds from interbonded poly(e-caprolactone) fibrous matrices with controlled porosity
Author(s) Tang, Yanwei
Wong, Cynthia
Wang, HongxiaORCID iD for Wang, Hongxia
Sutti, AlessandraORCID iD for Sutti, Alessandra
Kirkland, Mark
Wang, XungaiORCID iD for Wang, Xungai
Lin, TongORCID iD for Lin, Tong
Journal name Tissue engineering. Part C. methods
Volume number 17
Issue number 2
Start page 209
End page 217
Total pages 9
Publisher Mary Ann Liebert Publishers
Place of publication New Rochelle, N.Y.
Publication date 2011-02
ISSN 1937-3384
Summary In this article, we report on the preparation and cell culture performance of a novel fibrous matrix that has an interbonded fiber architecture, excellent pore interconnectivity, and controlled pore size and porosity. The fibrous matrices were prepared by combining melt-bonding of short synthetic fibers with a template leaching technique. The microcomputed tomography and scanning electron microscopy imaging verified that the fibers in the matrix were highly bonded, forming unique isotropic pore architectures. The average pore size and porosity of the fibrous matrices were controlled by the fiber/template ratio. The matrices having the average pore size of 120, 207, 813, and 994 mm, with the respective porosity of 73%, 88%, 96%, and 97%, were investigated. The applicability of the matrix as a three-dimensional (3D) tissue scaffold for cell culture was demonstrated with two cell lines, rat skin fibroblast and Chinese hamster ovary, and the influences of the matrix porosity and surface area on the cell culture performance were examined. Both cell lines grew successfully in the matrices, but they showed different preferences in pore size and porosity. Compared with two-dimensional tissue culture plates, the cell number on 3D fibrous matrices was increased by 97.27% for the Chinese hamster ovary cells and 49.46% for the fibroblasts after 21 days of culture. The fibroblasts in the matrices not only grew along the fiber surface but also bridged among the fibers, which was much different from those on two-dimensional scaffolds. Such an interbonded fibrous matrix may be useful for developing new fiber-based 3D tissue scaffolds for various cell culture applications.
Language eng
DOI 10.1089/ten.tec.2010.0223
Field of Research 100404 Regenerative Medicine (incl Stem Cells and Tissue Engineering)
Socio Economic Objective 870303 Polymeric Materials (e.g. Paints)
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2010
Copyright notice ©2010, Mary Ann Liebert Publishers
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Document type: Journal Article
Collections: Centre for Material and Fibre Innovation
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