Akt, AS160, metabolic risk factors and aerobic fitness in middle-aged women Levinger, Itamar, Howlett, Kirsten F., Peake, Jonathan, Garnham, Andrew, Hare, David L., Jerums, George, Selig, Steve and Goodman, Craig 2010, Akt, AS160, metabolic risk factors and aerobic fitness in middle-aged women, Exercise immunology review, vol. 16, pp. 98-104. Attached Files Name Description MIMEType Size Downloads selig-aktas160metabolic-2010.pdf Published version application/pdf 202.65KB 231 Title Akt, AS160, metabolic risk factors and aerobic fitness in middle-aged women Author(s) Levinger, Itamar Howlett, Kirsten F. orcid.org/0000-0002-8571-4867 Peake, Jonathan Garnham, Andrew Hare, David L. Jerums, George Selig, Steve orcid.org/0000-0003-4159-5111 Goodman, Craig Journal name Exercise immunology review Volume number 16 Start page 98 End page 104 Total pages 7 Publisher Human Kinetics Place of publication Champaign, Ill. Publication date 2010 ISSN 1077-5552 Keyword(s) aerobic fitness cytokines inflammation insulin signaling Summary This study investigated the association between the basal (rest) insulin-signaling proteins, Akt, and the Akt substrate AS160, metabolic risk factors, inflammatory markers and aerobic fitness, in middle-aged women with varying numbers of metabolic risk factors for type 2 diabetes. Methods: Sixteen women (n=16) aged 51.3±5.1 (mean ±SD) years provided muscle biopsies and blood samples at rest. In addition, anthropometric characteristics and aerobic power were assessed and the number of metabolic risk factors for each participant was determined (IDF criteria). Results: The mean number of metabolic risk factors was 1.6±1.2. Total Akt was negatively correlated with IL-1β (r = -0.45, p = 0.046), IL-6 (r = -0.44, p = 0.052) and TNF-α (r = -0.51, p = 0.025). Phosphorylated AS160 was positively correlated with HDL (r = 0.58, p= 0.024) and aerobic fitness (r = 0.51, p=0.047). Furthermore, a multiple regression analysis revealed that both HDL (t=2.5, p=0.032) and VO<sub>2peak</sub> (t=2.4, p=0.037) were better predictor for phosphorylated AS160 than TNF-α or IL-6 (p>0.05). Conclusions: Elevated inflammatory markers and increased metabolic risk factors may inhibit insulin-signaling protein phosphorylation in middle-aged women, thereby increasing insulin resistance under basal conditions. Furthermore, higher HDL and fitness levels are associated with an increase AS160 phosphorylation, which may in turn reduce insulin resistance. Notes Reproduced with the specific permission of the copyright owner. Language eng Field of Research 119999 Medical and Health Sciences not elsewhere classified Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences HERDC Research category C1.1 Refereed article in a scholarly journal Copyright notice ©2010, Human Kinetics Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30033425 Document type: Journal Article Collections: Faculty of Health School of Exercise and Nutrition Sciences Institute for Physical Activity and Nutrition Open Access Collection   Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Wed, 16 Mar 2011, 15:00:06 EST Tue, 03 May 2011, 09:57:12 EST Tue, 03 May 2011, 09:58:26 EST Wed, 04 May 2011, 14:15:28 EST Thu, 05 May 2011, 11:39:05 EST Wed, 15 Jun 2011, 13:43:36 EST Tue, 19 Jul 2011, 13:02:35 EST Tue, 01 Nov 2011, 14:49:50 EST Wed, 21 Oct 2015, 17:14:18 EST Tue, 06 Sep 2016, 16:42:49 EST Tue, 05 Dec 2017, 10:48:16 EST Fri, 07 Sep 2018, 15:19:57 EST Filtered Full Citation counts:   Cited 12 times in TR Web of Science Cited 12 times in Scopus Search Google Scholar Access Statistics: 942 Abstract Views, 231 File Downloads  -  Detailed Statistics Created: Wed, 16 Mar 2011, 15:00:06 EST