Insulin, a key regulator of hormone responsive milk protein synthesis during lactogenesis in murine mammary explants

Menzies, Karensa K., Lee, Heather J., Lefevre, Christophe, Ormandy, Christopher J., Macmillan, Keith L. and Nicholas, Kevin R. 2010, Insulin, a key regulator of hormone responsive milk protein synthesis during lactogenesis in murine mammary explants, Functional and integrative genomics, vol. 10, no. 1, pp. 87-95, doi: 10.1007/s10142-009-0140-0.

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Title Insulin, a key regulator of hormone responsive milk protein synthesis during lactogenesis in murine mammary explants
Author(s) Menzies, Karensa K.
Lee, Heather J.
Lefevre, Christophe
Ormandy, Christopher J.
Macmillan, Keith L.
Nicholas, Kevin R.
Journal name Functional and integrative genomics
Volume number 10
Issue number 1
Start page 87
End page 95
Total pages 9
Publisher Springer
Place of publication Heidelberg, Germany
Publication date 2010-03
ISSN 1438-793X
Keyword(s) Elf5
milk protein
mammary gland
Summary Murine milk protein gene expression requires insulin, hydrocortisone, and prolactin; however, the role of insulin is not well understood. This study, therefore, examined the requirement of insulin for milk protein synthesis. Mammary explants were cultured in various combinations of the lactogenic hormones and global changes in gene expression analysed using Affymetrix microarray. The expression of 164 genes was responsive to insulin, and 18 were involved in protein synthesis at the level of transcription and posttranscription, as well as amino acid uptake and metabolism. The folate receptor gene was increased by fivefold, highlighting a potentially important role for the hormone in folate metabolism, a process that is emerging to be central for protein synthesis. Interestingly, gene expression of two milk protein transcription factors, Stat5a and Elf5, previously identified as key components of prolactin signalling, both showed an essential requirement for insulin. Subsequent experiments in HCll cells confirmed that Stat5a and Elf5 gene expression could be induced in the absence of prolactin but in the presence of insulin. Whereas prolactin plays an essential role in phosphorylating and activating Stat5a, gene expression is only induced when insulin is present. This indicates insulin plays a crucial role in the transcription of the milk protein genes.
Language eng
DOI 10.1007/s10142-009-0140-0
Field of Research 060399 Evolutionary Biology not elsewhere classified
Socio Economic Objective 830507 Unprocessed or Minimally Processed Milk
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2010
Copyright notice ©2009, Springer-Verlag
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