Acetylene plasma polymerized surfaces for covalent immobilization of dense bioactive protein monolayers Yin, Yongbai, Bilek, Marcela M. M., McKenzie, David R., Nosworthy, Neil J., Kondyurin, Alexey, Youssef, Hani, Byrom, Michael J. and Yang, Wenrong 2009, Acetylene plasma polymerized surfaces for covalent immobilization of dense bioactive protein monolayers, Surface and coatings technology, vol. 203, no. 10-11, pp. 1310-1316, doi: 10.1016/j.surfcoat.2008.10.035. Attached Files Name Description MIMEType Size Downloads Title Acetylene plasma polymerized surfaces for covalent immobilization of dense bioactive protein monolayers Author(s) Yin, Yongbai Bilek, Marcela M. M. McKenzie, David R. Nosworthy, Neil J. Kondyurin, Alexey Youssef, Hani Byrom, Michael J. Yang, Wenrong orcid.org/0000-0001-8815-1951 Journal name Surface and coatings technology Volume number 203 Issue number 10-11 Start page 1310 End page 1316 Total pages 7 Publisher Elsevier B. V. Place of publication Amsterdam, The Netherlands Publication date 2009-02-25 ISSN 0257-8972 Keyword(s) plasma polymerization protein immobilization covalent attachment quartz crystal microbalance FTIR ellipsometer Summary Smooth polymerized surfaces, suitable for biochemical and biomedical applications, were deposited using a modified plasma enhanced chemical vapour deposition method with acetylene as a reaction precursor. Horseradish peroxidase (HRP) activity assays showed that the protein immobilized on the plasma polymerized surfaces maintained its biological function for a much longer period of time compared to that on uncoated surfaces. The kinetics of HRP attachment to the plasma polymerized surfaces were analyzed using quartz crystal microbalance with dissipation analysis. Spectroscopic ellipsometry and attenuated total reflection Fourier transform infrared spectroscopy were used to determine the thickness and the quantity of the attached protein. The results showed that the plasma polymerized surfaces provided a high density of attachment sites to covalently immobilize a dense monolayer of proteins. Language eng DOI 10.1016/j.surfcoat.2008.10.035 Field of Research 100703 Nanobiotechnology Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences HERDC Research category C1.1 Refereed article in a scholarly journal Copyright notice ©2008, Elsevier B.V. Persistent URL http://hdl.handle.net/10536/DRO/DU:30034967 Document type: Journal Article Collections: Institute for Technology Research and Innovation GTP Research Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Mon, 30 May 2011, 10:55:07 EST Fri, 16 Sep 2011, 13:44:18 EST Fri, 16 Sep 2011, 13:45:25 EST Thu, 11 Dec 2014, 17:03:35 EST Filtered Full Citation counts:   Cited 41 times in TR Web of Science Cited 46 times in Scopus Search Google Scholar Access Statistics: 798 Abstract Views, 2 File Downloads  -  Detailed Statistics Created: Mon, 30 May 2011, 10:55:07 EST