Highly selective and sensitive DNA assay based on electrocatalytic oxidation of ferrocene bearing zinc(II)-cyclen complexes with diethylamine

Shiddiky, Muhammad J. A., Torriero, Angel A. J., Zeng, Zhanghua, Spiccia, Leone and Bond, Alan M. 2010, Highly selective and sensitive DNA assay based on electrocatalytic oxidation of ferrocene bearing zinc(II)-cyclen complexes with diethylamine, Journal of the American chemical society, vol. 132, no. 29, pp. 10053-10063, doi: 10.1021/ja1021365.

Attached Files
Name Description MIMEType Size Downloads

Title Highly selective and sensitive DNA assay based on electrocatalytic oxidation of ferrocene bearing zinc(II)-cyclen complexes with diethylamine
Author(s) Shiddiky, Muhammad J. A.
Torriero, Angel A. J.ORCID iD for Torriero, Angel A. J. orcid.org/0000-0001-8616-365X
Zeng, Zhanghua
Spiccia, Leone
Bond, Alan M.
Journal name Journal of the American chemical society
Volume number 132
Issue number 29
Start page 10053
End page 10063
Total pages 11
Publisher American Chemical Society
Place of publication Washington, D.C.
Publication date 2010-07-02
ISSN 0002-7863
1520-5126
Summary A highly selective and sensitive electrochemical biosensor has been developed that detects DNA hybridization by employing the electrocatalytic activity of ferrocene (Fc) bearing cyclen complexes (cyclen = 1,4,7,10-tetraazacyclododecane, Fc[Zn(cyclen)H2O]2(ClO4)4 (R1), Fc(cyclen)2 (R2), Fc[Zn(cyclen)H2O](ClO4)2 (R3), and Fc(cyclen) (R4)). A sandwich-type approach, which involves hybridization of a target probe hybridized with the preimmobilized thiolated capture probe attached to a gold electrode, is employed to fabricate a DNA duplex layer. Electrochemical signals are generated by voltammetric interrogation of a Fc bearing Zn−cyclen complexes that selectively and quantitatively binds to the duplex layers through strong chelation between the cyclen complexes and particular nucleobases within the DNA sequence. Chelate formation between R1 or R3 and thymine bases leads to the perturbation of base-pair (A−T) stacking in the duplex structure, which greatly diminishes the yield of DNA-mediated charge transport and displays a marked selectivity to the presence of the target DNA sequence. Coupling the redox chemistry of the surface-bound Fc bearing Zn−cyclen complex and dimethylamine provides an electrocatalytic pathway that increases sensitivity of the assay and allows the 100 fM target DNA sequence to be detected. Excellent selectivity against even single-base sequence mismatches is achieved, and the DNA sensor is stable and reusable.
Language eng
DOI 10.1021/ja1021365
Field of Research 030604 Electrochemistry
030102 Electroanalytical Chemistry
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2010, American Chemical Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30038932

Document type: Journal Article
Collections: Centre for Material and Fibre Innovation
GTP Research
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 51 times in TR Web of Science
Scopus Citation Count Cited 50 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 598 Abstract Views, 1 File Downloads  -  Detailed Statistics
Created: Mon, 24 Oct 2011, 07:52:20 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.