Properties of an in vitro selected Pb2+ cleavage motif

Pan, Tao, Dichtl, Bernhard and Uhlenbeck, Olke C. 1994, Properties of an in vitro selected Pb2+ cleavage motif, Biochemistry, vol. 33, no. 32, pp. 9561-9565, doi: 10.1021/bi00198a023.

Attached Files
Name Description MIMEType Size Downloads

Title Properties of an in vitro selected Pb2+ cleavage motif
Author(s) Pan, Tao
Dichtl, BernhardORCID iD for Dichtl, Bernhard
Uhlenbeck, Olke C.
Journal name Biochemistry
Volume number 33
Issue number 32
Start page 9561
End page 9565
Total pages 5
Publisher American Chemical Society
Place of publication Washington, D. C.
Publication date 1994-08
ISSN 0006-2960
Keyword(s) chemical reaction
drug effect
protein folding
rna cleavage
rna structure
site directed mutagenesis
Summary The addition of Pb 2+ to a small RNA molecule consisting of an asymmetric internal loop of six nucleotides results in site-specific cleavage followed by hydrolysis of the 2′,3′-cyclic phosphate intermediate [Pan, T., & Uhlenbeck, O. C. (1992) Nature 358, 560-563]. Here we show that the reaction is highly specific for Pb 2+ and the cleavage rate increases exponentially with pH from 5.5 to 7.0, both in the presence and in the absence of Mg 2+. This suggests that the reaction mechanism involves Pb 2+ hydroxide acting as a base. Several sequence variants of the RNA are found to be equally active in both steps of the reaction, suggesting that they fold into a similar structure.
Language eng
DOI 10.1021/bi00198a023
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©1994, American Chemical Society
Persistent URL

Connect to link resolver
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 82 times in TR Web of Science
Scopus Citation Count Cited 76 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 1006 Abstract Views, 1 File Downloads  -  Detailed Statistics
Created: Mon, 24 Oct 2011, 14:15:59 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact