Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination

Dichtl, Bernhard, Blank, Diana, Sadowski, Martin, Hubner, Wolfgang, Weiser, Stefan and Keller, Walter 2002, Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination, EMBO journal, vol. 21, no. 15, pp. 4125-4135, doi: 10.1093/emboj/cdf390.

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Title Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination
Author(s) Dichtl, BernhardORCID iD for Dichtl, Bernhard orcid.org/0000-0001-5514-4982
Blank, Diana
Sadowski, Martin
Hubner, Wolfgang
Weiser, Stefan
Keller, Walter
Journal name EMBO journal
Volume number 21
Issue number 15
Start page 4125
End page 4135
Total pages 11
Publisher Nature Publishing Group
Place of publication London
Publication date 2002-08-01
ISSN 0261-4189
Keyword(s) ß-propeller repeats
pre-mRNA 3'-end formation
RNA polymerase II C-terminal domain
RNA–protein interactions
transcription termination
Summary RNA polymerase II (pol II) transcription termination requires co-transcriptional recognition of a functional polyadenylation signal, but the molecular mechanisms that transduce this signal to pol II remain unclear. We show that Yhh1p/Cft1p, the yeast homologue of the mammalian AAUAAA interacting protein CPSF 160, is an RNA-binding protein and provide evidence that it participates in poly(A) site recognition. Interestingly, RNA binding is mediated by a central domain composed of predicted -propeller-forming repeats, which occurs in proteins of diverse cellular functions. We also found that Yhh1p/Cft1p bound specifically to the phosphorylated C-terminal domain (CTD) of pol II in vitro and in a two-hybrid test in vivo. Furthermore, transcriptional run-on analysis demonstrated that yhh1 mutants were defective in transcription termination, suggesting that Yhh1p/Cft1p functions in the coupling of transcription and 3'-end formation. We propose that direct interactions of Yhh1p/Cft1p with both the RNA transcript and the CTD are required to communicate poly(A) site recognition to elongating pol II to initiate transcription termination.
Language eng
DOI 10.1093/emboj/cdf390
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2002, Nature Publishing Group
Persistent URL http://hdl.handle.net/10536/DRO/DU:30039331

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