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A single PDZ domain protein interacts with the menkes copper ATPase, ATP7A : a new protein implicated in copper homeostasis

Stephenson, Sarah E. M., Dubach, Daphne, Lim, Chris M., Mercer, Julian F. B. and La Fontaine, Sharon. 2005, A single PDZ domain protein interacts with the menkes copper ATPase, ATP7A : a new protein implicated in copper homeostasis, Journal of biological chemistry, vol. 280, no. 39, pp. 33270-33279, doi: 10.1074/jbc.M505889200.

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Title A single PDZ domain protein interacts with the menkes copper ATPase, ATP7A : a new protein implicated in copper homeostasis
Author(s) Stephenson, Sarah E. M.
Dubach, Daphne
Lim, Chris M.ORCID iD for Lim, Chris M. orcid.org/0000-0002-5044-1539
Mercer, Julian F. B.
La Fontaine, Sharon.ORCID iD for La Fontaine, Sharon. orcid.org/0000-0002-9948-074X
Journal name Journal of biological chemistry
Volume number 280
Issue number 39
Start page 33270
End page 33279
Publisher American Society for Biochemistry and Molecular Biology
Place of publication Baltimore, Md
Publication date 2005-09-30
ISSN 0021-9258
1083-351X
Summary The homeostatic regulation of essential elements such as copper requires many proteins whose activities are often mediated and tightly coordinated through protein-protein interactions. This regulation ensures that cells receive enough copper without intracellular concentrations reaching toxic levels. To date, only a small number of proteins implicated in copper homeostasis have been identified, and little is known of the protein-protein interactions required for this process. To identify other proteins important for copper homeostasis, while also elucidating the protein-protein interactions that are integral to the process, we have utilized a known copper protein, the copper ATPase ATP7A, as a bait in a yeast two-hybrid screen of a human cDNA library to search for interacting partners. One of the ATP7A-interacting proteins identified is a novel protein with a single PDZ domain. This protein was recently identified to interact with the plasma membrane calcium ATPase b-splice variants. We propose a change in name for this protein from PISP (plasma membrane calcium ATPase-interacting single-PDZ protein) to AIPP1 (ATPase-interacting PDZ protein) and suggest that it represents the protein that interacts with the class I PDZ binding motif identified at the ATP7A C terminus. The interaction in mammalian cells was confirmed and an additional splice variant of AIPP1 was identified. This study represents an essential step forward in identifying the proteins and elucidating the network of protein-protein interactions involved in maintaining copper homeostasis and validates the use of the yeast two-hybrid approach for this purpose.
Language eng
DOI 10.1074/jbc.M505889200
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2005 by The American Society for Biochemistry and Molecular Biology, Inc.
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30039503

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.