Ultrastructural analysis of plastids in angiosperm pollen tubes

Staff, I. A., Taylor, P. E., Kenrick, J. and Knox, R. B. 1989, Ultrastructural analysis of plastids in angiosperm pollen tubes, Sexual plant reproduction, vol. 2, no. 2, pp. 70-76, doi: 10.1007/BF00191993.

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Title Ultrastructural analysis of plastids in angiosperm pollen tubes
Author(s) Staff, I. A.
Taylor, P. E.ORCID iD for Taylor, P. E. orcid.org/0000-0002-4204-1893
Kenrick, J.
Knox, R. B.
Journal name Sexual plant reproduction
Volume number 2
Issue number 2
Start page 70
End page 76
Total pages 7
Publisher Springer
Place of publication Heidelberg, Germany
Publication date 1989
ISSN 0934-0882
Keyword(s) pollen tube
tannic acid
Summary When Rhododendron pollen tubes are cultured in the dark, electron-dense bodies are present that appear to be a metabolically altered form of a proplastid that is difficult to fix for electron microscopy, and whose membranes may not be intact. When similar pollen tubes are cultured in a dark/light regime, ultrastructurally well-defined proplastids are present after fixation in glutaraldehyde with PIPES buffer and tannic acid, followed by osmic acid. This fixation technique also gave the best ultrastructural images of those proplastids in pollen tubes grown in the dark. Pollen tube plastids have the potential to become chromoplasts when cultured in a dark/light regime as evidenced by the presence of branched tubules characteristic of these organelles. Light appears to be a hitherto neglected environmental factor involved in regulating pollen tube growth. This improved fixation procedure demonstrates the bilayered nature of the membranes surrounding sperm cells and the existence of cytoplasmic channels connecting sperm cell and pollen tube plasma membranes.
Language eng
DOI 10.1007/BF00191993
Field of Research 059999 Environmental Sciences not elsewhere classified
Socio Economic Objective 970105 Expanding Knowledge in the Environmental Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30039871

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