Major grass pollen allergen Lol p 1 binds to diesel exhaust particles : implications for asthma and air pollution

Knox, R. B., Suphioglu, C., Taylor, P. E., Desai, R., Watson, H. C., Peng, J. L. and Bursil, L. A. 1997, Major grass pollen allergen Lol p 1 binds to diesel exhaust particles : implications for asthma and air pollution, Clinical and experimental allergy, vol. 27, no. 3, pp. 246-251, doi: 10.1111/j.1365-2222.1997.tb00702.x.

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Title Major grass pollen allergen Lol p 1 binds to diesel exhaust particles : implications for asthma and air pollution
Author(s) Knox, R. B.
Suphioglu, C.ORCID iD for Suphioglu, C.
Taylor, P. E.ORCID iD for Taylor, P. E.
Desai, R.
Watson, H. C.
Peng, J. L.
Bursil, L. A.
Journal name Clinical and experimental allergy
Volume number 27
Issue number 3
Start page 246
End page 251
Total pages 6
Publisher Wiley-Blackwell Publishing Ltd.
Place of publication West Sussex, England
Publication date 1997-03
ISSN 0954-7894
Keyword(s) grass pollen
fine particles
diesel exhaust
immunogold labelling
monoclonal antibody
Lol p 1
Summary Background Grass pollen allergens are known to be present in the atmosphere in a range of particle sizes from whole pollen grains (approx. 20 to 55 μim in diameter) to smaller size fractions < 2.5 μ (fine particles, PM2.5). These latter particles are within the respirable range and include allergen-containing starch granules released from within the grains into the atmosphere when grass pollen ruptures in rainfall and are associated with epidemics of thunderstorm asthma during the grass pollen season. The question arises whether grass pollen allergens can interact with other sources of fine particles, particularly those present during episodes of air pollution.

Objective We propose the hypothesis that free grass pollen allergen molecules, derived from dead or burst grains and dispersed in microdroplets of water in aerosols, can bind to fine particles in polluted air.

Methods We used diesel exhaust carbon particles (DECP) derived from the exhaust of a stationary diesel engine, natural highly purified Lol p 1, immunogold labelling with specific monoclonal antibodies and a high voltage transmission electron -microscopic imaging technique

Results DECP are visualized as small carbon spheres, each 30–60 nm in diameter, forming fractal aggregates about 1–2μ in diameter. Here we test our hypothesis and show by in vitro experiments that the major grass pollen allergen, Lol p I. binds to one defined class of fine particles, DECP.

Conclusion DECP are in the respirable size range, can bind to the major grass pollen allergen Lol p I under in vitro conditions and represent a possible mechanism by which allergens can become concentrated in polluted air and thus trigger attacks of asthma.
Language eng
DOI 10.1111/j.1365-2222.1997.tb00702.x
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
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