Conservation of the ST6Gal I gene and its expression in the mammary gland

Maksimovic, Jovana, Sharp, Julie A., Nicholas, Kevin R., Cocks, Benjamin G. and Savin, Keith 2011, Conservation of the ST6Gal I gene and its expression in the mammary gland, Glycobiology, vol. 21, no. 4, pp. 467-481.

Attached Files
Name Description MIMEType Size Downloads

Title Conservation of the ST6Gal I gene and its expression in the mammary gland
Author(s) Maksimovic, Jovana
Sharp, Julie A.ORCID iD for Sharp, Julie A.
Nicholas, Kevin R.
Cocks, Benjamin G.
Savin, Keith
Journal name Glycobiology
Volume number 21
Issue number 4
Start page 467
End page 481
Total pages 15
Publisher Oxford University Press
Place of publication Cary, N. C.
Publication date 2011
ISSN 0959-6658
Keyword(s) mammary gland
sialic acid
Summary Milk sialoglycoconjugates can protect the gastrointestinal tract of the suckling neonate by competitively binding to invading pathogens and promoting growth of beneficial flora, and their potential role in postnatal brain development is of particular interest in human infant nutrition. Although the concentration and the distribution of sialoglycoconjugates have been extensively studied in the milk of various species, the investigation of sialyltransferase gene expression in the mammary gland, in the context of lactation, has been limited. The sialyltransferase enzyme ST6Gal I transfers sialic acid from CMP-sialic acid to type 2 (Galβ1,4GlcNAc) free disaccharides or the termini of N- or O-linked oligosaccharides using an α2,6-linkage. Expression of the ST6Gal I gene is primarily regulated at the level of transcription through the use of several cell and development- specific promoters, producing transcripts with divergent 5′ untranslated regions (UTR). In the mouse mammary gland, the novel 5′UTR exon (L) appears to be associated with a drastic increase in ST6Gal I gene expression during lactation. We find that rats also possess an exon (L), suggesting conservation of this regulatory mechanism in rodents. In contrast, an exon (L)-containing transcript was not detected in the lactating bovine or human mammary gland. We also observed a trend of increasing ST6Gal I gene expression in the bovine mammary gland, culminating in involution. This is in contrast to species such as mice where the greatest change in ST6Gal I gene expression occurs between pregnancy and lactation, suggesting different roles in rodents vs. other mammals for α2,6-sialylated oligosaccharides present in milk.
Language eng
Field of Research 069999 Biological Sciences not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2010, The Author
Persistent URL

Document type: Journal Article
Collections: Centre for Material and Fibre Innovation
GTP Research
Connect to link resolver
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 10 times in TR Web of Science
Scopus Citation Count Cited 11 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 1354 Abstract Views, 5 File Downloads  -  Detailed Statistics
Created: Mon, 05 Dec 2011, 12:51:52 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact