RAP1 controls rhoptry targeting of RAP2 in the malaria parasite Plasmodium falciparum

Baldi, Deborah L., Andrews, Katherine T., Waller, Ross F., Roos, David S., Howard, Randell F., Crabb, Brendan S. and Cowman, Alan F. 2000, RAP1 controls rhoptry targeting of RAP2 in the malaria parasite Plasmodium falciparum, EMBO journal, vol. 19, no. 11, pp. 2435-2443, doi: 10.1093/emboj/19.11.2435.

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Title RAP1 controls rhoptry targeting of RAP2 in the malaria parasite Plasmodium falciparum
Formatted title RAP1 controls rhoptry targeting of RAP2 in the malaria parasite Plasmodium falciparum
Author(s) Baldi, Deborah L.
Andrews, Katherine T.
Waller, Ross F.
Roos, David S.
Howard, Randell F.
Crabb, Brendan S.
Cowman, Alan F.
Journal name EMBO journal
Volume number 19
Issue number 11
Start page 2435
End page 2443
Publisher Nature Publishing Group
Place of publication London, England
Publication date 2000
ISSN 0261-4189
Keyword(s) malaria
molecular parasitology
targeted gene disruption
vaccine antigens
Summary Rhoptry associated protein 1 (RAP1) and 2 (RAP2), together with a poorly described third protein RAP3, form the low molecular weight complex within the rhoptries of Plasmodium falciparum. These proteins are thought to play a role in erythrocyte invasion by the extracellular merozoite and are important vaccine candidates. We used gene-targeting technology in P.falciparum blood-stage parasites to disrupt the RAP1 gene, producing parasites that express severely truncated forms of RAP1. Immunoprecipitation experiments suggest that truncated RAP1 species did not complex with RAP2 and RAP3. Consistent with this were the distinct subcellular localizations of RAP1 and 2 in disrupted RAP1 parasites, where RAP2 does not traffic to the rhoptries but is instead located in a compartment that appears related to the lumen of the endoplasmic reticulum. These results suggest that RAP1 is required to localize RAP2 to the rhoptries, supporting the hypothesis that rhoptry biogenesis is dependent in part on the secretory pathway in the parasite. The observation that apparently host-protective merozoite antigens are not essential for efficient erythrocyte invasion has important implications for vaccine design.
Language eng
DOI 10.1093/emboj/19.11.2435
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2000, European Molecular Biology Organization
Persistent URL http://hdl.handle.net/10536/DRO/DU:30041063

Document type: Journal Article
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