Antibacterial activity of stevioside towards food-borne pathogenic bacteria

Puri, Munish and Sharma, Deepika 2011, Antibacterial activity of stevioside towards food-borne pathogenic bacteria, Engineering in life sciences, vol. 11, no. 3, pp. 326-329.

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Title Antibacterial activity of stevioside towards food-borne pathogenic bacteria
Author(s) Puri, MunishORCID iD for Puri, Munish
Sharma, Deepika
Journal name Engineering in life sciences
Volume number 11
Issue number 3
Start page 326
End page 329
Total pages 4
Publisher Wiley - VCH Verlag GmbH & Co. KGaA
Place of publication Berlin, Germany
Publication date 2011-06
ISSN 1618-0240
Keyword(s) food borne
NMR spectroscopy
solvent extraction
Summary This study determines the inhibitory effect of Stevia rebaudiana leaf extracts and its purified bioactive compound ‘stevioside’ against food-related pathogens. The S. rebaudiana solvent extracts (1000 μg/mL) displayed antibacterial activity to Serratia marcescens, Klebsiella pneumoniae, Bacillus cereus, Pseudomonas aeruginosa, B. subtilis, Alcaligenes denitrificans and Salmonella typhimurium. Of the six solvents, ethanol and acetone extracts displayed the highest zone of inhibition. The bioactive compound from S. rebaudiana was purified by solvent extraction, thin-layer chromatography followed by structural characterization by spectroscopy evidence. Purified stevioside prevented the growth of tested bacterial species, i.e. B. subtilis, K. pneumoniae and S. typhimurium. Significant zone of inhibition (12 mm) was observed against B. cereus which proposes potential application of stevioside in foods to increase their shelf life.
Language eng
Field of Research 100301 Biocatalysis and Enzyme Technology
Socio Economic Objective 970110 Expanding Knowledge in Technology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2011, WILEY-VCH Verlag GmbH & Co. KGaA
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