Effects of exercise on the isoelectric patterns of erythropoietin Lamon, Severine, Martin, Laurent, Robinson, Neil, Saugy, Martial, de Ceaurriz, Jacques and Lasne, Francoise 2009, Effects of exercise on the isoelectric patterns of erythropoietin, Clinical journal of sport medicine, vol. 19, no. 4, pp. 311-315, doi: 10.1097/JSM.0b013e3181a9483e. Attached Files Name Description MIMEType Size Downloads Title Effects of exercise on the isoelectric patterns of erythropoietin Author(s) Lamon, Severine orcid.org/0000-0002-3271-6551 Martin, Laurent Robinson, Neil Saugy, Martial de Ceaurriz, Jacques Lasne, Francoise Journal name Clinical journal of sport medicine Volume number 19 Issue number 4 Start page 311 End page 315 Total pages 5 Publisher Lippincott Williams & Wilkins Place of publication Philadelphia, Pa. Publication date 2009-07 ISSN 1050-642X 1536-3724 Keyword(s) effort erythropoietin doping urine Summary Objectives: Recombinant erythropoietin has a strong impact on aerobic power and is therefore one of the most potent doping agents in endurance sports. The anti-doping control of this synthetic hormone relies on the detection, in the urine, of its isoelectric pattern, which differs from that of the corresponding natural hormone, the latter being typically more acidic than the former. However, a small number of natural urinary patterns, referred to as atypical patterns, are less acidic than the dominant form. Based on anecdotal evidence, the occurrence of such patterns seems to be related to particular strenuous exercises. This study aimed to demonstrate this relation using a strenuous exercise protocol. Design: Seven athletes took part in a training protocol including a series of supramaximal short-duration exercises. Urine and blood samples were collected throughout the protocols. Settings: World Cycling Center, Aigle, Switzerland, and research laboratories. Participants: Seven top-level athletes (cyclists) were involved in this study. Main Outcome Measures: Erythropoietin (EPO) isoelectric patterns were obtained by submitting blood and urine samples to isoelectric focusing. Additional protein dosages were performed. Results: Supramaximal short-duration exercises induced the transformation of typical urinary natural EPO patterns into atypical ones. None of the obtained atypical patterns fulfilled the 3 criteria mandatory for reporting an adverse analytical finding. Serum EPO patterns were not affected by the exercises that caused the transformation of urinary patterns. Conclusion: An exercise-induced transient renal dysfunction is proposed as a hypothetic explanation for these observations that rely on parallel investigations of proteinuria in the same samples. Language eng DOI 10.1097/JSM.0b013e3181a9483e Field of Research 119999 Medical and Health Sciences not elsewhere classified Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences HERDC Research category C1.1 Refereed article in a scholarly journal Persistent URL http://hdl.handle.net/10536/DRO/DU:30045285 Document type: Journal Article Collections: Faculty of Health School of Exercise and Nutrition Sciences Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Mon, 14 May 2012, 15:08:29 EST Tue, 05 Feb 2013, 09:22:24 EST Tue, 05 Feb 2013, 09:25:04 EST Fri, 13 Jun 2014, 14:06:19 EST Thu, 11 Dec 2014, 17:49:11 EST Filtered Full Citation counts:   Cited 24 times in TR Web of Science Cited 25 times in Scopus Search Google Scholar Access Statistics: 709 Abstract Views, 2 File Downloads  -  Detailed Statistics Created: Mon, 14 May 2012, 15:08:29 EST