Self-assembly of functional, amphipathic amyloid monolayers by the fungal hydrophobin EAS Macindoe, Ingrid, Kwan, Ann H., Ren, Qin, Morris, Vanessa K., Yang, Wenrong, Mackay, Joel P. and Sunde, Margaret 2012, Self-assembly of functional, amphipathic amyloid monolayers by the fungal hydrophobin EAS, National Academy of Sciences. Proceedings, vol. 109, no. 14, pp. E804-E811, doi: 10.1073/pnas.1114052109. Attached Files Name Description MIMEType Size Downloads Title Self-assembly of functional, amphipathic amyloid monolayers by the fungal hydrophobin EAS Author(s) Macindoe, Ingrid Kwan, Ann H. Ren, Qin Morris, Vanessa K. Yang, Wenrong orcid.org/0000-0001-8815-1951 Mackay, Joel P. Sunde, Margaret Journal name National Academy of Sciences. Proceedings Volume number 109 Issue number 14 Start page E804 End page E811 Total pages 8 Publisher National Academy of Sciences Place of publication Washington, D. C. Publication date 2012-04-03 ISSN 0027-8424 1091-6490 Keyword(s) fibril formation proteins surface aggregation sequence nanostructures prediction mechanism peptides insights Summary The hydrophobin EAS from the fungus Neurospora crassa forms functional amyloid fibrils called rodlets that facilitate spore formation and dispersal. Self-assembly of EAS into fibrillar rodlets occurs spontaneously at hydrophobic:hydrophilic interfaces and the rodlets further associate laterally to form amphipathic monolayers. We have used site-directed mutagenesis and peptide experiments to identify the region of EAS that drives intermolecular association and formation of the cross-β rodlet structure. Transplanting this region into a nonamyloidogenic hydrophobin enables it to form rodlets. We have also determined the structure and dynamics of an EAS variant with reduced rodlet-forming ability. Taken together, these data allow us to pinpoint the conformational changes that take place when hydrophobins self-assemble at an interface and to propose a model for the amphipathic EAS rodlet structure. Language eng DOI 10.1073/pnas.1114052109 Field of Research 069999 Biological Sciences not elsewhere classified Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences HERDC Research category C1 Refereed article in a scholarly journal Persistent URL http://hdl.handle.net/10536/DRO/DU:30047006 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Life and Environmental Sciences Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Mon, 13 Aug 2012, 12:59:24 EST Tue, 23 Oct 2012, 11:42:47 EST Tue, 23 Oct 2012, 11:43:37 EST Fri, 13 Jun 2014, 14:44:43 EST Fri, 08 Nov 2019, 08:41:06 EST Sun, 20 Mar 2022, 01:36:41 EST Filtered Full Citation counts:   Cited 89 times in TR Web of Science Cited 93 times in Scopus Search Google Scholar Access Statistics: 638 Abstract Views, 6 File Downloads  -  Detailed Statistics Created: Mon, 13 Aug 2012, 12:59:24 EST