The type II secretion system and its ubiquitous lipoprotein substrate, SsIE are required for biofilm formation and virulence of enteropathogenic escherichia coli Baldi, Deborah L., Higginson, Ellen E., Hocking, Dianna M., Praszkier, Judyta, Cavaliere, Rosalia, James, Catherine E., Bennett-Wood, Vicki, Azzopardi, Kristy I., Turnbull, Lynne, Lithgow, Trevor, Robins-Browne, Roy M., Whitchurch, Cynthia B. and Tauschek, Marija 2012, The type II secretion system and its ubiquitous lipoprotein substrate, SsIE are required for biofilm formation and virulence of enteropathogenic escherichia coli, Infection and immunity, vol. 80, no. 6, pp. 2042-2052, doi: 10.1128/IAI.06160-11. Attached Files Name Description MIMEType Size Downloads baldi-thetype-2012.pdf Published version application/pdf 2.97MB 305 Title The type II secretion system and its ubiquitous lipoprotein substrate, SsIE are required for biofilm formation and virulence of enteropathogenic escherichia coli Author(s) Baldi, Deborah L. orcid.org/0000-0001-5457-3400 Higginson, Ellen E. Hocking, Dianna M. Praszkier, Judyta Cavaliere, Rosalia James, Catherine E. Bennett-Wood, Vicki Azzopardi, Kristy I. Turnbull, Lynne Lithgow, Trevor Robins-Browne, Roy M. Whitchurch, Cynthia B. Tauschek, Marija Journal name Infection and immunity Volume number 80 Issue number 6 Start page 2042 End page 2052 Total pages 11 Publisher American Society for Microbiology Place of publication Washington, D. C. Publication date 2012-06 ISSN 0019-9567 1098-5522 Keyword(s) animals biofilms cell membrane enteropathogenic escherichia coli escherichia coli proteins gene expression regulation bacterial mutation rabbits substrate specificity virulence Summary Enteropathogenic Escherichia coli (EPEC) is a major cause of diarrhea in infants in developing countries. We have identified a functional type II secretion system (T2SS) in EPEC that is homologous to the pathway responsible for the secretion of heat-labile enterotoxin by enterotoxigenic E. coli. The wild-type EPEC T2SS was able to secrete a heat-labile enterotoxin reporter, but an isogenic T2SS mutant could not. We showed that the major substrate of the T2SS in EPEC is SslE, an outer membrane lipoprotein (formerly known as YghJ), and that a functional T2SS is essential for biofilm formation by EPEC. T2SS and SslE mutants were arrested at the microcolony stage of biofilm formation, suggesting that the T2SS is involved in the development of mature biofilms and that SslE is a dominant effector of biofilm development. Moreover, the T2SS was required for virulence, as infection of rabbits with a rabbit-specific EPEC strain carrying a mutation in either the T2SS or SslE resulted in significantly reduced intestinal colonization and milder disease. Language eng DOI 10.1128/IAI.06160-11 Field of Research 110801 Medical Bacteriology Socio Economic Objective 920109 Infectious Diseases HERDC Research category C1.1 Refereed article in a scholarly journal Copyright notice ©2012, American Society for Microbiology Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30047263 Document type: Journal Article Collections: Faculty of Health School of Medicine Open Access Collection Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Tue, 21 Aug 2012, 10:05:57 EST Fri, 24 Aug 2012, 16:11:54 EST Fri, 24 Aug 2012, 16:14:04 EST Fri, 13 Jun 2014, 14:55:06 EST Thu, 11 Dec 2014, 17:57:37 EST Mon, 02 Mar 2015, 12:56:49 EST Tue, 06 Sep 2016, 19:32:54 EST Tue, 05 Dec 2017, 12:35:04 EST Fri, 07 Sep 2018, 16:07:37 EST Filtered Full Citation counts:   Cited 60 times in TR Web of Science Cited 62 times in Scopus Search Google Scholar Access Statistics: 588 Abstract Views, 305 File Downloads  -  Detailed Statistics Created: Tue, 21 Aug 2012, 10:05:36 EST by Jane Moschetti