Role of Pr160gag-pol in mediating the selective incorporation of tRNA(Lys) into human immunodeficiency virus type 1 particles Mak, Johnson, Jiang, Min, Wainberg, Mark A., Hammarskjöld, Marie-Louise, Rekosh, David and Kleiman, Lawrence 1994, Role of Pr160gag-pol in mediating the selective incorporation of tRNA(Lys) into human immunodeficiency virus type 1 particles, Journal of virology, vol. 68, no. 4, pp. 2065-2072. Attached Files Name Description MIMEType Size Downloads mak-roleofpr160-1994.pdf Published version application/pdf 2.45MB 146 Title Role of Pr160gag-pol in mediating the selective incorporation of tRNA(Lys) into human immunodeficiency virus type 1 particles Formatted title  Role of Pr160gag-pol in mediating the selective incorporation of tRNALys into human immunodeficiency virus type 1 particles Author(s) Mak, Johnson orcid.org/0000-0002-5229-5707 Jiang, Min Wainberg, Mark A. Hammarskjöld, Marie-Louise Rekosh, David Kleiman, Lawrence Journal name Journal of virology Volume number 68 Issue number 4 Start page 2065 End page 2072 Total pages 8 Publisher American Society for Microbiology Place of publication Washington, D. C. Publication date 1994-04 ISSN 0022-538X 1098-5514 Keyword(s) HIV-1 tRNA RNA sequencing techniques Pr160gag-pol Summary COS-7 cells transfected with human immunodeficiency virus type 1 (HIV-1) proviral DNA produce virus in which three tRNA species are most abundant in the viral tRNA population. These tRNAs have been identified through RNA sequencing techniques as tRNA(3Lys) the primer tRNA in HIV-1, and members of the tRNA(1,2Lys) isoacceptor family. These RNAs represent 60% of the low-molecular-weight RNA isolated from virus particles, while they represent only 6% of the low-molecular-weight RNA isolated from the COS cell cytoplasm. Thus, tRNA(Lys) is selectively incorporated into HIV-1 particles. We have measured the ratio of tRNA(3Lys) molecules to copies of genomic RNA in viral RNA samples and have calculated that HIV-1 contains approximately eight molecules of tRNA(3Lys) per two copies of genomic RNA. We have also obtained evidence that the Pr160gag-pol precursor is involved in primer tRNA(3Lys) incorporation into virus. First, selective tRNA(Lys) incorporation and wild-type amounts of tRNA(3Lys) were maintained in a protease-negative virus unable to process Pr55gag and Pr160gag-pol precursors, indicating that precursor processing was not required for primer tRNA incorporation. Second, viral particles containing only unprocessed Pr55gag protein did not selectively incorporate tRNA(Lys), while virions containing both unprocessed Pr55gag and Pr160gag-pol proteins demonstrated select tRNA(3Lys) packaging. Third, studies with a proviral mutant containing a deletion of most of the reverse transcriptase sequences and approximately one-third of the integrase sequence in the Pr160gag-pol precursor resulted in the loss of selective tRNA incorporation and an eightfold decrease in the amount of tRNA(3Lys) per two copies of genomic RNA. We have also confirmed herein finding of a previous study which indicated that the primer binding site is not required for the selective incorporation of tRNA(Lys). Language eng Field of Research 119999 Medical and Health Sciences not elsewhere classified Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences HERDC Research category C1.1 Refereed article in a scholarly journal Copyright notice ©1994, American Society for Microbiology Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30047523 Document type: Journal Article Collections: Faculty of Health School of Medicine Open Access Collection Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Thu, 30 Aug 2012, 09:38:51 EST Thu, 30 Aug 2012, 10:37:02 EST Tue, 18 Sep 2012, 09:49:44 EST Tue, 18 Sep 2012, 09:51:04 EST Thu, 30 Jan 2014, 14:39:45 EST Fri, 13 Jun 2014, 15:07:51 EST Tue, 06 Sep 2016, 19:36:21 EST Sat, 17 Jun 2017, 03:12:42 EST Sat, 21 Oct 2017, 02:28:01 EST Tue, 05 Dec 2017, 12:35:17 EST Fri, 07 Sep 2018, 16:08:08 EST Filtered Full Citation counts:   Cited 175 times in TR Web of Science Cited 165 times in Scopus Search Google Scholar Access Statistics: 435 Abstract Views, 146 File Downloads  -  Detailed Statistics Created: Thu, 30 Aug 2012, 09:38:51 EST