Sex reversal in Nile tilapia (Oreochromis niloticus Linnaeus) by androgen immersion

Wassermann, Gustavo Javier and Afonso, Luís Orlando Bertolla 2003, Sex reversal in Nile tilapia (Oreochromis niloticus Linnaeus) by androgen immersion, Aquaculture research, vol. 34, no. 1, pp. 65-71, doi: 10.1046/j.1365-2109.2003.00795.x.

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Title Sex reversal in Nile tilapia (Oreochromis niloticus Linnaeus) by androgen immersion
Author(s) Wassermann, Gustavo Javier
Afonso, Luís Orlando BertollaORCID iD for Afonso, Luís Orlando Bertolla
Journal name Aquaculture research
Volume number 34
Issue number 1
Start page 65
End page 71
Total pages 7
Publisher Wiley - Blackwell Publishing
Place of publication Oxford, England
Publication date 2003-01
ISSN 1355-557X
Keyword(s) masculinization
sex control
Summary Two experiments were conducted to investigate the efficacy of three androgens applied through immersion treatments on the sex ratio of Nile tilapia (Oreochromis niloticus L.) fry. In experiment 1, 14 days post-hatching (DPH) larvae were exposed to a single immersion treatment in 17α-methyltestosterone (MT), 17α-methyldihydrotestosterone (MDHT) or 17α-ethynyltestosterone (ET) at 200, 600 and 1800 μg L−1 over 4 h (130 larvae per treatment). In experiment 2, Nile tilapia larvae were exposed to the higher androgen concentration (1800 μg L−1) applied as either a single immersion (14 DPH) or double immersion (10 and 14 DPH) over 4 h (125 larvae per treatment). Change in sex proportion within each experiment as well as between experiments was analysed by the chi-square test. In experiment 1, MT, MDHT and ET were equally effective in significantly increasing the proportion of males when applied at 1800 μg L−1 (86.0%, 90.0% and 86.7% respectively). At 200 μg L−1 none of the androgens altered sex ratio. At 600 μg L−1, only MDHT slightly, but significantly skewed the sex ratio towards males (73.0%). In experiment 2, a single immersion treatment at 14 DPH (1800 μg L−1) significantly increased the proportion of males, but at this time the response was significantly hormone dependent (MDHT, 100.0%; MT, 91.6%; ET, 76.9%). When compared with a single immersion, two-immersion treatments significantly increased the proportion of males in the MT-treated group (from 91.6% to 98.3%), decreased the proportion of males in the MDHT group (from 100.0% to 93.4%) and had no significant effect the ET-treated group (change from 76.9% to 82.5%). The overall comparison of the sex ratio among same treatments from different experiments (a single immersion in 1800 μg L−1) was not significantly different.
Language eng
DOI 10.1046/j.1365-2109.2003.00795.x
Field of Research 070401 Aquaculture
070405 Fish Physiology and Genetics
Socio Economic Objective 830102 Aquaculture Fin Fish (excl. Tuna)
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2003, Wiley-Blackwell Publishing
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