Ex vivo expansion of haematopoietic stem/progenitor cells from human umbilical cord blood on acellular scaffolds prepared from MS-5 stromal cell line Tiwari, Abhilasha, Tursky, Melinda L., Mushahary, Dolly, Wasnik, Samiksha, Collier, Fiona M., Suma, Kantipudi, Kirkland, Mark A. and Pande, Gopal 2012, Ex vivo expansion of haematopoietic stem/progenitor cells from human umbilical cord blood on acellular scaffolds prepared from MS-5 stromal cell line, Journal of tissue engineering and regenerative medicine, vol. 7, pp. 871-883, doi: 10.1002/term.1479. Attached Files Name Description MIMEType Size Downloads Title Ex vivo expansion of haematopoietic stem/progenitor cells from human umbilical cord blood on acellular scaffolds prepared from MS-5 stromal cell line Author(s) Tiwari, Abhilasha Tursky, Melinda L. Mushahary, Dolly Wasnik, Samiksha Collier, Fiona M. orcid.org/0000-0002-5438-480X Suma, Kantipudi Kirkland, Mark A. Pande, Gopal Journal name Journal of tissue engineering and regenerative medicine Volume number 7 Start page 871 End page 883 Total pages 13 Publisher John Wiley and Sons Place of publication Hoboken, New Jersey Publication date 2012 ISSN 1932-6254 1932-7005 Keyword(s) acellular biological scaffold extracellular matrix haematopoietic niche proteomics stem cell expansion umbilical cord blood Summary Lineage-specific expansion of haematopoietic stem/progenitor cells (HSPCs) from human umbilical cord blood (UCB) is desirable because of their several applications in translational medicine, e.g. treatment of cancer, bonemarrowfailure and immunodeficiencies. The currentmethods forHSPC expansion use either cellular feeder layers and/or soluble growth factors and selected matrix components coated on different surfaces. The use of cell-free extracellular matrices from bone marrow cells for this purpose has not previously been reported. We have prepared insoluble, cell- free matrices from a murine bone marrow stromal cell line (MS-5) grown under four different conditions, i.e. in presence or absence of osteogenic medium, each incubated under 5% and 20% O2 tensions. These acellularmatrices were used as biological scaffolds for the lineage-specific expansion of magnetically sorted CD34+ cells and the results were evaluated by flow cytometry and colony-forming assays. We could get up to 80-fold expansion of some HSPCs on one of the matrices and our results indicated that oxygen tension played a significant role in determining the expansion capacity of the matrices. A comparative proteomic analysis of the matrices indicated differential expression of proteins, such as aldehyde dehydrogenase and gelsolin, which have previously been identified as playing a role in HSPC maintenance and expansion. Our approach may be of value in identifying factors relevant to tissue engineering-based ex vivo HSPC expansion, and itmay also provide insights into the constitution of the niche in which these cells reside in the bone marrow. Language eng DOI 10.1002/term.1479 Field of Research 060103 Cell Development, Proliferation and Death 060106 Cellular Interactions (incl Adhesion, Matrix, Cell Wall) 060109 Proteomics and Intermolecular Interactions (excl Medical Proteomics) Socio Economic Objective 920101 Blood Disorders HERDC Research category C1.1 Refereed article in a scholarly journal Copyright notice ©2012, John Wiley and Sons Persistent URL http://hdl.handle.net/10536/DRO/DU:30047854 Document type: Journal Article Collections: Institute for Frontier Materials GTP Research Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Mon, 03 Sep 2012, 14:09:56 EST Thu, 04 Oct 2012, 09:10:24 EST Thu, 04 Oct 2012, 09:12:52 EST Tue, 04 Dec 2012, 13:16:24 EST Tue, 04 Dec 2012, 13:29:43 EST Fri, 11 Jan 2013, 15:24:51 EST Wed, 17 Apr 2013, 10:58:20 EST Fri, 09 Aug 2013, 13:07:04 EST Tue, 05 Nov 2013, 08:14:10 EST Tue, 05 Nov 2013, 08:16:36 EST Tue, 05 Nov 2013, 08:17:39 EST Tue, 05 Nov 2013, 10:27:27 EST Mon, 11 Nov 2013, 08:35:34 EST Mon, 11 Nov 2013, 08:36:17 EST Fri, 22 Aug 2014, 16:12:34 EST Mon, 20 Oct 2014, 14:39:47 EST Tue, 03 Feb 2015, 13:15:12 EST Filtered Full Citation counts:   Cited 27 times in TR Web of Science Cited 30 times in Scopus Search Google Scholar Access Statistics: 449 Abstract Views, 9 File Downloads  -  Detailed Statistics Created: Mon, 03 Sep 2012, 14:09:56 EST