Effects of dietary selenium on post-ischemic expression of antioxidant mRNA

Venardos, Kylie, Ashton, Kevin, Headrick, John and Perkins, Anthony 2005, Effects of dietary selenium on post-ischemic expression of antioxidant mRNA, Molecular and cellular biochemistry, vol. 270, no. 1-2, pp. 131-138.

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Title Effects of dietary selenium on post-ischemic expression of antioxidant mRNA
Author(s) Venardos, Kylie
Ashton, Kevin
Headrick, John
Perkins, Anthony
Journal name Molecular and cellular biochemistry
Volume number 270
Issue number 1-2
Start page 131
End page 138
Total pages 8
Publisher Springer New York LLC
Place of publication Secaucus, N. J.
Publication date 2005-02
ISSN 0300-8177
Keyword(s) glutathione peroxidase
mRNA expression
thioredoxin reductase
Summary Cardiac ischemia reperfusion leads to oxidative stress and poor physiological recovery. Selenium deficiency down-regulates thioredoxin reductase (Txnrd) and glutathione peroxidase (Gpx) activity, impairing recovery from ischemia-reperfusion. Furthermore, selenium supplementation has been shown to be cardioprotective and lessens oxidative stress in reperfused rat hearts. In this study we have investigated the role of selenium in the mRNA expression of these, and related antioxidant proteins, post ischemia-reperfusion. Male rats were fed varying doses of selenium for five weeks. Hearts were isolated and perfused using the Langendorff method with 22.5 min of global ischemia and 45 min reperfusion. RNA was extracted for quantitative real-time PCR analysis of glutathione peroxidase (Gpx)-1 and 4, glutathione reductase (Gsr), thioredoxin peroxidase-2 (Prdx2), thioredoxin (Txn) and thioredoxin reductase (Txnrd)-1 and 2 gene expression. Selenium deficiency produced significant reductions in Gpx-1, Gpx-4, Prdx2, Txnrd-1 and Txnrd-2 expression. Conversely, selenium supplementation of 1000 μg/kg significantly up-regulated Gpx-1, Gpx-4, Txn, Txnrd-1 and Txnrd-2 transcription. Our results show selenium modulates the cardiac mRNA expression of thioredoxin and glutathione related enzymes post ischemia-reperfusion, and impacts on tolerance to ischemia-reperfusion.
Language eng
Field of Research 069999 Biological Sciences not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2005, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30048035

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