An alternative for the extraction and storage of DNA from insects in forensic entomology

Harvey, Michelle L. 2005, An alternative for the extraction and storage of DNA from insects in forensic entomology, Journal of forensic sciences, vol. 50, no. 3, pp. 1-3, doi: 10.1520/JFS2004404.

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Title An alternative for the extraction and storage of DNA from insects in forensic entomology
Author(s) Harvey, Michelle L.ORCID iD for Harvey, Michelle L.
Journal name Journal of forensic sciences
Volume number 50
Issue number 3
Start page 1
End page 3
Total pages 3
Publisher Wiley-Blackwell Publishing
Place of publication London, England
Publication date 2005-05
ISSN 0022-1198
Keyword(s) forensic science
forensic entomology
Whatman FTA
Summary An important area of recent research in forensic entomology has been the use of insect DNA to provide identification of insects for fast and accurate estimation of time since death. This requires DNA to be extracted efficiently and in a state suitable for use in molecular procedures, and then stored on a long-term basis. In this study, Whatman FTA™ cards were tested for use with the Calliphoridae (Diptera). In particular, testing examined their ability to effectively extract DNA from specimens, and store and provide DNA template in a suitable condition for amplification using the polymerase chain reaction (PCR). The cards provided DNA that was able to be amplified from a variety of life stages, and thus appears to be of sufficient quality and quantity for use in subsequent procedures. FTA cards therefore appear suitable for use with calliphorids, and provide a new method of extraction that is simple and efficient and allows for storage and transportation without refrigeration, consequently simplifying the handling of DNA in forensic entomological cases.
Language eng
DOI 10.1520/JFS2004404
Field of Research 069901 Forensic Biology
Socio Economic Objective 940403 Criminal Justice
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2005, ASTM
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