Differentiated mTOR but not AMPK signaling after strength vs endurance exercise in training-accustomed individuals

Vissing, K., McGee, S. L., Farup, J., Kjolhede, T., Vendelbo, M. H. and Jessen, N. 2013, Differentiated mTOR but not AMPK signaling after strength vs endurance exercise in training-accustomed individuals, Scandinavian journal of medicine and science in sports, vol. 23, no. 3, pp. 355-366, doi: 10.1111/j.1600-0838.2011.01395.x.

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Title Differentiated mTOR but not AMPK signaling after strength vs endurance exercise in training-accustomed individuals
Author(s) Vissing, K.
McGee, S. L.ORCID iD for McGee, S. L. orcid.org/0000-0001-6953-106X
Farup, J.
Kjolhede, T.
Vendelbo, M. H.
Jessen, N.
Journal name Scandinavian journal of medicine and science in sports
Volume number 23
Issue number 3
Start page 355
End page 366
Total pages 12
Publisher John Wiley & Sons
Place of publication Malden, Mass.
Publication date 2013-06
ISSN 0905-7188
Keyword(s) resistance exercise
protein synthesis
hypertrophy signaling
acute response
AMPK substrates
Summary The influence of adenosine mono phosphate (AMP)-activated protein kinase (AMPK) vs Akt-mammalian target of rapamycin C1 (mTORC1) protein signaling mechanisms on converting differentiated exercise into training specific adaptations is not well-established. To investigate this, human subjects were divided into endurance, strength, and non-exercise control groups. Data were obtained before and during post-exercise recovery from single-bout exercise, conducted with an exercise mode to which the exercise subjects were accustomed through 10 weeks of prior training. Blood and muscle samples were analyzed for plasma substrates and hormones and for muscle markers of AMPK and Akt-mTORC1 protein signaling. Increases in plasma glucose, insulin, growth hormone (GH), and insulin-like growth factor (IGF)-1, and in phosphorylated muscle phospho-Akt substrate (PAS) of 160 kDa, mTOR, 70 kDa ribosomal protein S6 kinase, eukaryotic initiation factor 4E, and glycogen synthase kinase 3α were observed after strength exercise. Increased phosphorylation of AMPK, histone deacetylase5 (HDAC5), cAMP response element-binding protein, and acetyl-CoA carboxylase (ACC) was observed after endurance exercise, but not differently from after strength exercise. No changes in protein phosphorylation were observed in non-exercise controls. Endurance training produced an increase in maximal oxygen uptake and a decrease in submaximal exercise heart rate, while strength training produced increases in muscle cross-sectional area and strength. No changes in basal levels of signaling proteins were observed in response to training. The results support that in training-accustomed individuals, mTORC1 signaling is preferentially activated after hypertrophy-inducing exercise, while AMPK signaling is less specific for differentiated exercise.
Language eng
DOI 10.1111/j.1600-0838.2011.01395.x
Field of Research 111699 Medical Physiology not elsewhere classified
Socio Economic Objective 920104 Diabetes
HERDC Research category C1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30050101

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
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