Orally administered [14C]DPA and [14C]DHA are metabolised differently to [14C]EPA in rats Kaur, Gunveen, Molero, Juan C., Weisinger, Harrison S. and Sinclair, Andrew J. 2013, Orally administered [14C]DPA and [14C]DHA are metabolised differently to [14C]EPA in rats, British journal of nutrition, vol. 109, no. 3, pp. 441-448, doi: 10.1017/S0007114512001419. Attached Files Name Description MIMEType Size Downloads Title Orally administered [14C]DPA and [14C]DHA are metabolised differently to [14C]EPA in rats Author(s) Kaur, Gunveen orcid.org/0000-0002-6250-0495 Molero, Juan C. Weisinger, Harrison S. orcid.org/0000-0001-6044-3653 Sinclair, Andrew J. Journal name British journal of nutrition Volume number 109 Issue number 3 Start page 441 End page 448 Total pages 8 Publisher Cambridge University Press Place of publication Cambridge, England Publication date 2013-02 ISSN 0007-1145 1475-2662 Keyword(s) n-3 PUFA docosapentaenoic acid EPA DHA b-oxidation Summary Previous studies have revealed that C20 PUFA are significantly less oxidised to CO2 in whole-body studies compared with SFA, MUFA and C18 PUFA. The present study determined the extent to which three long-chain PUFA, namely 20 : 5n-3 EPA, 22 : 5n-3 docosapentaenoic acid (DPA) and 22 : 6n-3 DHA, were catabolised to CO2 or, conversely, incorporated into tissue lipids. Rats were administered a single oral dose of 2·5 μCi [1-14C]DPA, [1-14C]EPA, [1-14C]DHA or [1-14C]oleic acid (18 : 1n-9; OA), and were placed in a metabolism chamber for 6 h where exhaled 14CO2 was trapped and counted for radioactivity. Rats were euthanised after 24 h and tissues were removed for analysis of radioactivity in tissue lipids. The results showed that DPA and DHA were catabolised to CO2 significantly less compared with EPA and OA (P< 0·05). The phospholipid (PL) fraction was the most labelled for all three n-3 PUFA compared with OA in all tissues, and there was no difference between C20 and C22 n-3 PUFA in the proportion of label in the PL fraction. The DHA and DPA groups showed significantly more label than the EPA group in both skeletal muscle and heart. In the brain and heart tissue, there was significantly less label in the cholesterol fraction from the C22 n-3 PUFA group compared with the C20 n-3 PUFA group. The higher incorporation of DHA and DPA into the heart and skeletal muscle, compared with EPA, suggests that these C22 n-3 PUFA might play an important role in these tissues. Language eng DOI 10.1017/S0007114512001419 Field of Research 111103 Nutritional Physiology Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2012, The Authors Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30050693 Document type: Journal Article Collections: Faculty of Health School of Medicine Open Access Collection Related Links Link Description Link to full-text (open access)   Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Tue, 19 Feb 2013, 13:05:15 EST Tue, 19 Feb 2013, 13:12:02 EST Wed, 20 Feb 2013, 09:14:58 EST Wed, 20 Feb 2013, 09:15:35 EST Wed, 20 Feb 2013, 09:21:22 EST Mon, 16 Dec 2013, 15:57:09 EST Fri, 13 Jun 2014, 16:37:41 EST Mon, 19 Jan 2015, 11:52:52 EST Fri, 08 Nov 2019, 14:39:17 EST Filtered Full Citation counts:   Cited 19 times in TR Web of Science Cited 22 times in Scopus Search Google Scholar Access Statistics: 667 Abstract Views, 4 File Downloads  -  Detailed Statistics Created: Tue, 19 Feb 2013, 13:05:13 EST by Jane Moschetti