Electroretinography in streptozotocin diabetic rats following acute intraocular pressure elevation Kohzaki, Kenichi, Vingrys, Algis J., Armitage, James A. and Bui, Bang V. 2013, Electroretinography in streptozotocin diabetic rats following acute intraocular pressure elevation, Basic science, vol. 251, no. 2, pp. 529-535, doi: 10.1007/s00417-012-2212-4. Attached Files Name Description MIMEType Size Downloads Title Electroretinography in streptozotocin diabetic rats following acute intraocular pressure elevation Author(s) Kohzaki, Kenichi Vingrys, Algis J. Armitage, James A. orcid.org/0000-0002-3762-0911 Bui, Bang V. Journal name Basic science Volume number 251 Issue number 2 Start page 529 End page 535 Total pages 7 Publisher Springer Place of publication Heidelberg, Germany Publication date 2013-02 ISSN 0721-832X Keyword(s) Electroretinogram Intraocular pressure Diabetes Hyperglycemia Streptozotocin Rat Retina Summary Background We consider whether pre-existing streptozotocin induced hyperglycemia in rats affects the ability of the eye to cope with a single episode of acute intraocular pressure (IOP) elevation. Methods Electroretinogram (ERG) responses were measured (−6.08 to 1.92 log cd.s.m−2) in anaesthetized (60:5 mg/kg ketamine:xylazine) dark-adapted (>12 h) adult Sprague–Dawley rats 1 week after a single acute IOP elevation to 70 mmHg for 60 min. This was undertaken in rats treated 11 weeks earlier with streptozotocin (STZ, n = 12, 50 mg/kg at 6 weeks of age) or citrate buffer (n = 12). ERG responses were analyzed to derive an index of photoreceptor (a-wave), ON-bipolar (b-wave), amacrine (oscillatory potentials) and inner retinal (positive scotopic threshold response, pSTR) function. Results One week following acute IOP elevation there was a significant reduction of the ganglion cell pSTR (−35 ± 11 %, P = 0.0161) in STZ-injected animals. In contrast the pSTR in citrate-injected animals was not significant changed (+16 ± 14 %). The negative component of the STR was unaffected by IOP elevation in either citrate or STZ-treated groups. Photoreceptoral (a-wave, citrate-control +4 ± 3 %, STZ +4 ± 5 %) and ON-bipolar cell (b-wave, control +4 ± 3 %, STZ +4 ± 5 %) mediated responses were not significantly affected by IOP elevation in either citrate- or STZ-injected rats. Finally, oscillatory potentials (citrate-control +8 ± 23 %, STZ +1 ± 17 %) were not reduced 1 week after IOP challenge. Conclusions The ganglion cell dominated pSTR was reduced following a single episode of IOP elevation in STZ diabetic, but not control rats. These data indicate that hyperglycemia renders the inner retina more susceptible to IOP elevation. Language eng DOI 10.1007/s00417-012-2212-4 Field of Research 111303 Vision Science Socio Economic Objective 920107 Hearing, Vision, Speech and Their Disorders HERDC Research category C1.1 Refereed article in a scholarly journal Persistent URL http://hdl.handle.net/10536/DRO/DU:30050742 Document type: Journal Article Collections: Faculty of Health School of Medicine Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Thu, 21 Feb 2013, 10:55:35 EST Wed, 27 Feb 2013, 17:12:41 EST Tue, 05 Mar 2013, 08:19:01 EST Tue, 05 Mar 2013, 08:19:58 EST Thu, 15 May 2014, 09:48:37 EST Thu, 11 Dec 2014, 18:32:19 EST Tue, 03 Feb 2015, 13:22:54 EST Filtered Full Citation counts:   Cited 3 times in TR Web of Science Cited 3 times in Scopus Search Google Scholar Access Statistics: 672 Abstract Views, 2 File Downloads  -  Detailed Statistics Created: Thu, 21 Feb 2013, 10:55:21 EST by Jane Moschetti