Utilising silk fibroin membranes as scaffolds for the growth of tympanic membrane keratinocytes, and application to myringoplasty surgery Levin, B., Redmond, S.L., Rajkhowa, R., Eikelboom, R.H., Atlas, M.D. and Marano, R.J. 2013, Utilising silk fibroin membranes as scaffolds for the growth of tympanic membrane keratinocytes, and application to myringoplasty surgery, Journal of laryngology and otology, vol. 127, no. Supp 1., pp. S13-S20, doi: 10.1017/S0022215112001661. Attached Files Name Description MIMEType Size Downloads Title Utilising silk fibroin membranes as scaffolds for the growth of tympanic membrane keratinocytes, and application to myringoplasty surgery Author(s) Levin, B. Redmond, S.L. Rajkhowa, R. orcid.org/0000-0002-6811-9126 Eikelboom, R.H. Atlas, M.D. Marano, R.J. Journal name Journal of laryngology and otology Volume number 127 Issue number Supp 1. Start page S13 End page S20 Total pages 8 Publisher Cambridge University Press Place of publication Cambridge, England Publication date 2013-01 ISSN 0022-2151 Keyword(s) tympanic membrane perforation myringoplasty silk fibroin graft keratinocyte Summary Background: Chronic tympanic membrane perforations can cause significant morbidity. The term myringoplasty describes the operation used to close such perforations. A variety of graft materials are available for use in myringoplasty, but all have limitations and few studies report post-operative hearing outcomes. Recently, the biomedical applications of silk fibroin protein have been studied. This material’s biocompatibility, biodegradability and ability to act as a scaffold to support cell growth prompted an investigation of its interaction with human tympanic membrane keratinocytes. Methods and materials: Silk fibroin membranes were prepared and human tympanic membrane keratinocytes cultured. Keratinocytes were seeded onto the membranes and immunostained for a number of relevant protein markers relating to cell proliferation, adhesion and specific epithelial differentiation. Results: The silk fibroin scaffolds successfully supported the growth and adhesion of keratinocytes, whilst also maintaining their cell lineage. Conclusion: The properties of silk fibroin make it an attractive option for further research, as a potential alternative graft in myringoplasty. Language eng DOI 10.1017/S0022215112001661 Field of Research 109999 Technology not elsewhere classified Socio Economic Objective 970110 Expanding Knowledge in Technology HERDC Research category C1 Refereed article in a scholarly journal Persistent URL http://hdl.handle.net/10536/DRO/DU:30051158 Document type: Journal Article Collections: Institute for Frontier Materials GTP Research Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Wed, 13 Mar 2013, 09:16:57 EST Wed, 13 Mar 2013, 10:36:34 EST Tue, 17 Dec 2013, 13:12:06 EST Tue, 17 Dec 2013, 13:13:50 EST Fri, 22 Aug 2014, 15:32:56 EST Filtered Full Citation counts:   Cited 19 times in TR Web of Science Cited 19 times in Scopus Search Google Scholar Access Statistics: 632 Abstract Views, 2 File Downloads  -  Detailed Statistics Created: Wed, 13 Mar 2013, 09:16:57 EST