The COMPASS subunit Spp1 links histone methylation to initiation of meiotic recombination

Acquaviva, Laurent, Szekvolgyi, Lorant, Dichtl, Berhard, Dichtl, Beatriz Solange, De, la Roche Saint Andre Christophe, Nicolas, Alain and Geli, Vincent 2013, The COMPASS subunit Spp1 links histone methylation to initiation of meiotic recombination, Science, vol. 339, no. 6116, pp. 215-218, doi: 10.1126/science.1225739.

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Title The COMPASS subunit Spp1 links histone methylation to initiation of meiotic recombination
Author(s) Acquaviva, Laurent
Szekvolgyi, Lorant
Dichtl, BerhardORCID iD for Dichtl, Berhard
Dichtl, Beatriz Solange
De, la Roche Saint Andre Christophe
Nicolas, Alain
Geli, Vincent
Journal name Science
Volume number 339
Issue number 6116
Start page 215
End page 218
Total pages 4
Publisher American Association for the Advancement of Science
Place of publication Washington, D.C.
Publication date 2013
ISSN 0036-8075
Summary During meiosis, combinatorial associations of genetic traits arise from homologous recombination between parental chromosomes. Histone H3 lysine 4 trimethylation marks meiotic recombination hotspots in yeast and mammals, but how this ubiquitous chromatin modification relates to the initiation of double-strand breaks (DSBs) dependent on Spo11 remains unknown. Here, we show that the tethering of a PHD-containing protein, Spp1 (a component of the COMPASS complex), to recombinationally cold regions is sufficient to induce DSB formation. Furthermore, we found that Spp1 physically interacts with Mer2, a key protein of the differentiated chromosomal axis required for DSB formation. Thus, by interacting with H3K4me3 and Mer2, Spp1 promotes recruitment of potential meiotic DSB sites to the chromosomal axis, allowing Spo11 cleavage at nearby nucleosome-depleted regions.
Language eng
DOI 10.1126/science.1225739
Field of Research 059999 Environmental Sciences not elsewhere classified
Socio Economic Objective 970105 Expanding Knowledge in the Environmental Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2013, American Association for the Advancement of Science
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