Polyglutamine tracts as modulators of transcriptional activation from yeast to mammals

Atanesyan, Lilit, Günther, Viola, Dichtl, Bernhard, Georgiev, Oleg and Schaffner, Walter 2012, Polyglutamine tracts as modulators of transcriptional activation from yeast to mammals, Biological chemistry, vol. 393, no. 1-2, pp. 63-70, doi: 10.1515/BC-2011-252.

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Title Polyglutamine tracts as modulators of transcriptional activation from yeast to mammals
Author(s) Atanesyan, Lilit
Günther, Viola
Dichtl, BernhardORCID iD for Dichtl, Bernhard orcid.org/0000-0001-5514-4982
Georgiev, Oleg
Schaffner, Walter
Journal name Biological chemistry
Volume number 393
Issue number 1-2
Start page 63
End page 70
Total pages 8
Publisher Walter de Gruyter GmbH & Co. KG
Place of publication Berlin, Germany
Publication date 2012-01-01
ISSN 1431-6730
Keyword(s) Drosophila
triplet repeat
transcription factor
Saccharomyces cerevisiae
polyglutamine repeat
Summary  Microsatellite repeats are genetically unstable and subject to expansion and shrinkage. A subset of them, triplet repeats, can occur within the coding region and specify homomeric tracts of amino acids. Polyglutamine (polyQ) tracts are enriched in eukaryotic regulatory proteins, notably transcription factors, and we had shown before that they can contribute to transcriptional activation in mammalian cells. Here we generalize this finding by also including evolutionarily divergent organisms, namely, Drosophila and baker's yeast. In all three systems, Gal4-based model transcription factors were more active if they harbored a polyQ tract, and the activity depended on the length of the tract. By contrast, a polyserine tract was inactive. PolyQs acted from either an internal or a C-terminal position, thus ruling out a merely structural 'linker' effect. Finally, a two-hybrid assay in mammalian cells showed that polyQ tracts can interact with each other, supporting the concept that a polyQ-containing transcription factor can recruit other factors with polyQ tracts or glutamine-rich activation domains. The widespread occurrence of polyQ repeats in regulatory proteins suggests a beneficial role; in addition to the contribution to transcriptional activity, their genetic instability might help a species to adapt to changing environmental conditions in a potentially reversible manner.
Language eng
DOI 10.1515/BC-2011-252
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30053075

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Created: Sun, 23 Jun 2013, 15:24:29 EST by Bernhard Dichtl

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