Profiling of secondary metabolites in blue lupin inoculated with Phytophthora cinnamomi following phosphite treatment

Gunning, Tiffany K., Conlan, Xavier A., Parker, Rhiannon M., Dyson, Gail A., Adams, Mike J., Barnett, Neil W. and Cahill, David M. 2013, Profiling of secondary metabolites in blue lupin inoculated with Phytophthora cinnamomi following phosphite treatment, Functional plant biology, vol. 40, no. 11, pp. 1089-1097, doi: 10.1071/FP13023.

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Title Profiling of secondary metabolites in blue lupin inoculated with Phytophthora cinnamomi following phosphite treatment
Author(s) Gunning, Tiffany K.ORCID iD for Gunning, Tiffany K.
Conlan, Xavier A.ORCID iD for Conlan, Xavier A.
Parker, Rhiannon M.
Dyson, Gail A.
Adams, Mike J.
Barnett, Neil W.
Cahill, David M.ORCID iD for Cahill, David M.
Journal name Functional plant biology
Volume number 40
Issue number 11
Start page 1089
End page 1097
Total pages 9
Publisher CSIRO Publishing
Place of publication Melbourne, Vic.
Publication date 2013
ISSN 1445-4408
Keyword(s) mass spectrometry
plant defence mechanisms
plant defense mechanisms
Summary In order to discover phytochemicals that are potentially bioactive against Phytophthora cinnamomi, (a soil-borne plant pathogen) a metabolite profiling protocol for investigation of metabolic changes in Lupinus angustifolius L. plant roots in response to pathogen challenge has been established. Analysis of the metabolic profiles from healthy and P. cinnamomi-inoculated root tissue with high resolution mass spectrometry and nuclear magnetic resonance spectroscopy confirmed that although susceptible, L. angustifolius upregulated a defence associated genistein and 2′-hydroxygenistein-based isoflavonoid and a soyasapogenol saponin at 12h post inoculation which increased in concentration at 72h post inoculation. In contrast to the typical susceptible interaction, the application of a phosphorous-based treatment to L. angustifolius foliage 48h before P. cinnamomi challenge negated the ability of the pathogen to colonise the root tissue and cause disease. Importantly, although the root profiles of water-treated and phosphite-treated plants post pathogen inoculation contained the same secondary metabolites, concentration variations were observed. Accumulation of secondary metabolites within the P. cinnamomi-inoculated plants confirms that pathogen ingress of the root interstitially occurs in phosphite-treated plants, confirming a direct mode of action against the pathogen upon breaching the root cells.
Language eng
DOI 10.1071/FP13023
Field of Research 069999 Biological Sciences not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2013, CSIRO Publishing
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