Metabolite profiling of biological extracts

Callahan, D. L., Bacic, A. and Roessner, U. 2009, Metabolite profiling of biological extracts, in ANZSMS 2009: 22nd Conference of the Australian and New Zealand Society for Mass Spectrometry 2009, Oxford University Press, [Sydney, N.S.W.], pp. 1-1.

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Title Metabolite profiling of biological extracts
Author(s) Callahan, D. L.ORCID iD for Callahan, D. L.
Bacic, A.
Roessner, U.
Conference name Australian and New Zealand Mass Spectrometry Society. Conference (2009 : Sydney, NSW)
Conference location Sydney, NSW
Conference dates 27-30 Jan. 2009
Title of proceedings ANZSMS 2009: 22nd Conference of the Australian and New Zealand Society for Mass Spectrometry 2009
Editor(s) [Unknown]
Publication date 2009
Conference series Australian and New Zealand Mass Spectrometry Society Conference
Start page 1
End page 1
Total pages 1
Publisher Oxford University Press
Place of publication [Sydney, N.S.W.]
Summary Metabolite profiling, HPLC, LC-QTOF-MS, GC-MS. A workflow will be presented for comprehensive metabolomics using LC- and GC-MS. Metabolomics is an emerging field in the suite of ‘omic’ approaches for Systems Biology. The goal of metabolomics is to detect the presence of all small-molecules in a biological sample. This presents a significant challenge due to the chemical diversity and large concentration range of metabolites. Currently, there is no single method which enables the entire metabolome to be analysed, therefore a suite of analytical approaches are required to increase the coverage of detected metabolites. The routinely used techniques for metabolite profiling are LC- and GC-MS and NMR. Here we present complementary approaches using MS hyphenated to different chromatographic techniques. GC-MS represent the most robust standardised technique for high throughput metabolite profiling however there are still no standard LC-based methods for profiling. Polar compounds represent the most challenging aspect of LC-based metabolomics. A robust chromatographic technique for profiling polar compounds using HILIC chromatography and QTOF-MS will be presented as well as the complimentary reverse phase LC-MS method. The polar separation was carried out using a diamond hydride column. This unique stationary phase provides stable retention times and fast re-equilibration which contrasts to other forms of HILIC stationary phases. These LC-based methods will be compared to the well established GC-MS method as well as NMRbased profiling.
Notes This paper was also published in Plant Cell Physiol. 49(5): 691–703 (2008) by Oxford University Press
Language eng
Field of Research 039999 Chemical Sciences not elsewhere classified
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category E1.1 Full written paper - refereed
Copyright notice ©2008, Oxford University Press
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