HIV-1 Infection of T cells and macrophages are differentially modulated by virion-associated Hck: a nef-dependent phenomenon Cornall, Alyssa, Mak, Johnson, Greenway, Alison and Tachedjian, Gilda 2013, HIV-1 Infection of T cells and macrophages are differentially modulated by virion-associated Hck: a nef-dependent phenomenon, Viruses, vol. 5, no. 9, pp. 2235-2252, doi: 10.3390/v5092235. Attached Files Name Description MIMEType Size Downloads mak-hiv1infection-2013.pdf Published version application/pdf 664.57KB 187 Title HIV-1 Infection of T cells and macrophages are differentially modulated by virion-associated Hck: a nef-dependent phenomenon Author(s) Cornall, Alyssa Mak, Johnson orcid.org/0000-0002-5229-5707 Greenway, Alison Tachedjian, Gilda Journal name Viruses Volume number 5 Issue number 9 Start page 2235 End page 2252 Total pages 18 Publisher M D P I AG Place of publication Basel, Switzerland Publication date 2013 ISSN 1999-4915 Keyword(s) HIV-1 Nef Hck macrophage T lymphocyte virion infectivity Summary The proline repeat motif (PxxP) of Nef is required for interaction with the SH3 domains of macrophage-specific Src kinase Hck. However, the implication of this interaction for viral replication and infectivity in macrophages and T lymphocytes remains unclear. Experiments in HIV-1 infected macrophages confirmed the presence of a Nef:Hck complex which was dependent on the Nef proline repeat motif. The proline repeat motif of Nef also enhanced both HIV-1 infection and replication in macrophages, and was required for incorporation of Hck into viral particles. Unexpectedly, wild-type Hck inhibited infection of macrophages, but Hck was shown to enhance infection of primary T lymphocytes. These results indicate that the interaction between Nef and Hck is important for Nef-dependent modulation of viral infectivity. Hck-dependent enhancement of HIV-1 infection of T cells suggests that Nef-Hck interaction may contribute to the spread of HIV-1 infection from macrophages to T cells by modulating events in the producer cell, virion and target cell. Language eng DOI 10.3390/v5092235 Field of Research 119999 Medical and Health Sciences not elsewhere classified Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences HERDC Research category C1 Refereed article in a scholarly journal Grant ID NHMRC 1025270 Copyright notice ©2013, M D P I AG Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30059526 Document type: Journal Article Collections: Faculty of Health School of Medicine Open Access Collection Related Links Link Description Connect to published version Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Thu, 16 Jan 2014, 10:01:27 EST Thu, 16 Jan 2014, 13:22:22 EST Fri, 17 Jan 2014, 14:05:51 EST Fri, 17 Jan 2014, 14:22:46 EST Fri, 17 Jan 2014, 14:36:21 EST Fri, 17 Jan 2014, 15:09:14 EST Fri, 17 Jan 2014, 15:10:57 EST Thu, 30 Jan 2014, 11:22:12 EST Mon, 12 May 2014, 15:33:10 EST Tue, 03 Feb 2015, 13:41:39 EST Tue, 05 Dec 2017, 12:46:27 EST Fri, 07 Sep 2018, 16:52:55 EST Filtered Full Citation counts:   Cited 5 times in TR Web of Science Cited 5 times in Scopus Search Google Scholar Access Statistics: 411 Abstract Views, 188 File Downloads  -  Detailed Statistics Created: Thu, 16 Jan 2014, 10:01:15 EST by Jane Moschetti