Skeletal muscle fat metabolism after exercise in humans: influence of fat availability

Kimber, Nicholas E, Cameron-Smith, David, McGee, Sean L and Hargreaves, Mark 2013, Skeletal muscle fat metabolism after exercise in humans: influence of fat availability, Journal of Applied Physiology, vol. 114, no. 11, pp. 1577-1585, doi: 10.1152/japplphysiol.00824.2012.

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Title Skeletal muscle fat metabolism after exercise in humans: influence of fat availability
Author(s) Kimber, Nicholas E
Cameron-Smith, David
McGee, Sean LORCID iD for McGee, Sean L
Hargreaves, Mark
Journal name Journal of Applied Physiology
Volume number 114
Issue number 11
Start page 1577
End page 1585
Total pages 9
Publisher American Physiological Society
Place of publication Bethesda, Md.
Publication date 2013
ISSN 8750-7587
Keyword(s) Fat availability
Intramuscular triglyceride
Postexercise recovery
Summary The mechanisms facilitating increased skeletal muscle fat oxidation following prolonged, strenuous exercise remain poorly defined. The aim of this study was to examine the influence of plasma free fatty acid (FFA) availability on intramuscular malonyl-CoA concentration and the regulation of whole-body fat metabolism during a 6-h postexercise recovery period. Eight endurance-trained men performed three trials, consisting of 1.5 h high-intensity and exhaustive exercise, followed by infusion of saline, saline + nicotinic acid (NA; low FFA), or Intralipid and heparin [high FFA (HFA)]. Muscle biopsies were obtained at the end of exercise (0 h) and at 3 and 6 h in recovery. Ingestion of NA suppressed the postexercise plasma FFA concentration throughout recovery (P < 0.01), except at 4 h. The alteration of the availability of plasma FFA during recovery induced a significant increase in whole-body fat oxidation during the 6-h period for HFA (52.2 ± 4.8 g) relative to NA (38.4 ± 3.1 g; P < 0.05); however, this response was unrelated to changes in skeletal muscle malonyl-CoA and acetyl-CoA carboxylase (ACC)β phosphorylation, suggesting mechanisms other than phosphorylation-mediated changes in ACC activity may have a role in regulating fat metabolism in human skeletal muscle during postexercise recovery. Despite marked changes in plasma FFA availability, no significant changes in intramuscular triglyceride concentrations were detected. These data suggest that the regulation of postexercise skeletal muscle fat oxidation in humans involves factors other than the 5′AMP-activated protein kinase-ACCβ-malonyl-CoA signaling pathway, although malonyl-CoA-mediated regulation cannot be excluded completely in the acute recovery period.
Language eng
DOI 10.1152/japplphysiol.00824.2012
Field of Research 111699 Medical Physiology not elsewhere classified
Socio Economic Objective 920104 Diabetes
HERDC Research category C1 Refereed article in a scholarly journal
Grant ID NHMRC 1030474
Copyright notice ©2013, American Physiological Society
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Document type: Journal Article
Collections: Faculty of Health
School of Medicine
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