The Nitric Oxide (NO)-Sensing Repressor NsrR of Neisseria meningitidis Has a Compact Regulon of Genes Involved in NO Synthesis and Detoxification Heurlier, Karin, Thomson, Melanie J, Aziz, Naveed and Moir, James W B 2008, The Nitric Oxide (NO)-Sensing Repressor NsrR of Neisseria meningitidis Has a Compact Regulon of Genes Involved in NO Synthesis and Detoxification, Journal of Bacteriology, vol. 190, no. 7, pp. 2488-2495, doi: 10.1128/JB.01869-07. Attached Files Name Description MIMEType Size Downloads Title The Nitric Oxide (NO)-Sensing Repressor NsrR of Neisseria meningitidis Has a Compact Regulon of Genes Involved in NO Synthesis and Detoxification Author(s) Heurlier, Karin Thomson, Melanie J Aziz, Naveed Moir, James W B Journal name Journal of Bacteriology Volume number 190 Issue number 7 Start page 2488 End page 2495 Total pages 8 Publisher American Society for Microbiology Place of publication Washington, DC Publication date 2008 ISSN 0021-9193 Keyword(s) Nitric oxide (NO)-Sensing repressor NsrR Neisseria meningitidis Summary We have analyzed the extent of regulation by the nitric oxide (NO)-sensitive repressor NsrR from Neisseria meningitidis MC58, using microarray analysis. Target genes that appeared to be regulated by NsrR, based on a comparison between an nsrR mutant and a wild-type strain, were further investigated by quantitative real-time PCR, revealing a very compact set of genes, as follows: norB (encoding NO reductase), dnrN (encoding a protein putatively involved in the repair of nitrosative damage to iron-sulfur clusters), aniA (encoding nitrite reductase), nirV (a putative nitrite reductase assembly protein), and mobA (a gene associated with molybdenum metabolism in other species but with a frame shift in N. meningitidis). In all cases, NsrR acts as a repressor. The NO protection systems norB and dnrN are regulated by NO in an NsrR-dependent manner, whereas the NO protection system cytochrome c′ (encoded by cycP) is not controlled by NO or NsrR, indicating that N. meningitidis expresses both constitutive and inducible NO protection systems. In addition, we present evidence to show that the anaerobic response regulator FNR is also sensitive to NO but less so than NsrR, resulting in complex regulation of promoters such as aniA, which is controlled by both FNR and NsrR: aniA was found to be maximally induced by intermediate NO concentrations, consistent with a regulatory system that allows expression during denitrification (in which NO accumulates) but is down-regulated as NO approaches toxic concentrations. Language eng DOI 10.1128/JB.01869-07 Field of Research 110309 Infectious Diseases 110303 Clinical Microbiology Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences HERDC Research category C1.1 Refereed article in a scholarly journal Persistent URL http://hdl.handle.net/10536/DRO/DU:30061410 Document type: Journal Article Collections: Faculty of Health School of Medicine Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Thu, 06 Mar 2014, 11:56:18 EST Fri, 14 Mar 2014, 09:10:52 EST Mon, 31 Mar 2014, 11:09:13 EST Thu, 11 Dec 2014, 19:57:21 EST Sat, 09 Nov 2019, 06:35:26 EST Sat, 12 Mar 2022, 19:37:57 EST Filtered Full Citation counts:   Cited 53 times in TR Web of Science Cited 51 times in Scopus Search Google Scholar Access Statistics: 279 Abstract Views, 1 File Downloads  -  Detailed Statistics Created: Thu, 06 Mar 2014, 11:56:18 EST