RNAi mediated Tiam1 gene knockdown inhibits invasion of retinoblastoma Subramanian, Nithya, Navaneethakrishnan, Saranya, Biswas, Jyotirmay, Kanwar, Rupinder K., Kanwar, Jagat R. and Krishnakumar, Subramanian 2013, RNAi mediated Tiam1 gene knockdown inhibits invasion of retinoblastoma, PLoS One, vol. 8, no. 8, pp. e70422, doi: 10.1371/journal.pone.0070422. Attached Files Name Description MIMEType Size Downloads kanwar-rnaimediated-2013.pdf Published version application/pdf 4.85MB 191 Title RNAi mediated Tiam1 gene knockdown inhibits invasion of retinoblastoma Author(s) Subramanian, Nithya Navaneethakrishnan, Saranya Biswas, Jyotirmay Kanwar, Rupinder K. Kanwar, Jagat R. orcid.org/0000-0003-3728-9568 Krishnakumar, Subramanian Journal name PLoS One Volume number 8 Issue number 8 Start page e70422 Total pages 14 Publisher PLoS Place of publication San Francisco, CA Publication date 2013-08 ISSN 1932-6203 Summary T lymphoma invasion and metastasis protein (Tiam1) is up-regulated in variety of cancers and its expression level is related to metastatic potential of the type of cancer. Earlier, Tiam1 was shown to be overexpressed in retinoblastoma (RB) and we hypothesized that it was involved in invasiveness of RB. This was tested by silencing Tiam1 in RB cell lines (Y79 and Weri-Rb1) using siRNA pool, targeting different regions of Tiam1 mRNA. The cDNA microarray of Tiam1 silenced cells showed gene regulations altered by Tiam1 were predominantly on the actin cytoskeleton interacting proteins, apoptotic initiators and tumorogenic potential targets. The silenced phenotype resulted in decreased growth and increased apoptosis with non-invasive characteristics. Transfection of full length and N-terminal truncated construct (C1199) clearly revealed membrane localization of Tiam1 and not in the case of C580 construct. F-actin staining showed the interaction of Tiam1 with actin in the membrane edges that leads to ruffling, and also imparts varying invasive potential to the cell. The results obtained from our study show for the first time that Tiam1 modulates the cell invasion, mediated by actin cytoskeleton remodeling in RB. Language eng DOI 10.1371/journal.pone.0070422 Field of Research 111204 Cancer Therapy (excl Chemotherapy and Radiation Therapy) 111207 Molecular Targets Socio Economic Objective 920102 Cancer and Related Disorders HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2013, PLoS Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30062764 Document type: Journal Article Collections: Faculty of Health School of Medicine Open Access Collection Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Wed, 30 Apr 2014, 16:44:40 EST Thu, 01 May 2014, 08:38:59 EST Wed, 07 May 2014, 08:23:06 EST Wed, 07 May 2014, 12:04:57 EST Wed, 07 May 2014, 12:09:41 EST Wed, 07 May 2014, 12:10:46 EST Thu, 29 May 2014, 14:56:03 EST Wed, 06 Dec 2017, 06:06:01 EST Fri, 07 Sep 2018, 17:05:13 EST Filtered Full Citation counts:   Cited 23 times in TR Web of Science Cited 22 times in Scopus Search Google Scholar Access Statistics: 625 Abstract Views, 191 File Downloads  -  Detailed Statistics Created: Wed, 30 Apr 2014, 16:44:24 EST by Rupinder Kanwar