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Identification of unprecedented anticancer properties of high molecular weight biomacromolecular complex containing bovine lactoferrin (HMW-bLf)

Ebrahim, F, Shankaranarayanan, JS, Kanwar, JR, Gurudevan, S, Krishnan, UM and Kanwar, RK 2014, Identification of unprecedented anticancer properties of high molecular weight biomacromolecular complex containing bovine lactoferrin (HMW-bLf), PLoS One, vol. 9, no. 9, e106568, pp. 1-13, doi: 10.1371/journal.pone.0106568.

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Title Identification of unprecedented anticancer properties of high molecular weight biomacromolecular complex containing bovine lactoferrin (HMW-bLf)
Author(s) Ebrahim, F
Shankaranarayanan, JS
Kanwar, JRORCID iD for Kanwar, JR orcid.org/0000-0003-3728-9568
Gurudevan, S
Krishnan, UM
Kanwar, RK
Journal name PLoS One
Volume number 9
Issue number 9
Season e106568
Start page 1
End page 13
Total pages 13
Publisher Public Library of Science (PLOS)
Place of publication San Francisco, CA
Publication date 2014-09
ISSN 1932-6203
Keyword(s) Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
STIMULATE OLIGOMERIZATION
THERMAL-DENATURATION
MILK LACTOFERRIN
IRON
CANCER
BINDING
DNA
APOPTOSIS
COLOSTRUM
OLIGOSACCHARIDES
Summary With the successful clinical trials, multifunctional glycoprotein bovine lactoferrin is gaining attention as a safe nutraceutical and biologic drug targeting cancer, chronic-inflammatory, viral and microbial diseases. Interestingly, recent findings that human lactoferrin oligomerizes under simulated physiological conditions signify the possible role of oligomerization in the multifunctional activities of lactoferrin molecule during infections and in disease targeting signaling pathways. Here we report the purification and physicochemical characterization of high molecular weight biomacromolecular complex containing bovine lactoferrin (≥250 kDa), from bovine colostrum, a naturally enriched source of lactoferrin. It showed structural similarities to native monomeric iron free (Apo) lactoferrin (∼78-80 kDa), retained anti-bovine lactoferrin antibody specific binding and displayed potential receptor binding properties when tested for cellular internalization. It further displayed higher thermal stability and better resistance to gut enzyme digestion than native bLf monomer. High molecular weight bovine lactoferrin was functionally bioactive and inhibited significantly the cell proliferation (p<0.01) of human breast and colon carcinoma derived cells. It induced significantly higher cancer cell death (apoptosis) and cytotoxicity in a dose-dependent manner in cancer cells than the normal intestinal cells. Upon cellular internalization, it led to the up-regulation of caspase-3 expression and degradation of actin. In order to identify the cutting edge future potential of this bio-macromolecule in medicine over the monomer, its in-depth structural and functional properties need to be investigated further.
Language eng
DOI 10.1371/journal.pone.0106568
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 920102 Cancer and Related Disorders
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Public Library of Science (PLOS)
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30067297

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Collection
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.