ADAMTS4 and ADAMTS5 knockout mice are protected from versican but not aggrecan or brevican proteolysis during spinal cord injury Demircan, K, Topcu, V, Takigawa, T, Akyol, S, Yonezawa, T, Ozturk, G, Ugurcu, V, Hasgul, R, Yigitoglu, M R, Akyol, O, McCulloch, D R and Hirohata, S 2014, ADAMTS4 and ADAMTS5 knockout mice are protected from versican but not aggrecan or brevican proteolysis during spinal cord injury, Biomed Research International, vol. 2014, Article number 693746, pp. 1-8, doi: 10.1155/2014/693746. Attached Files Name Description MIMEType Size Downloads SYMPLECTIC-LICENCE DRO_Licence_Agreement.txt text/plain 1.48KB 10 t022858-ADAMTS4-and-ADAMTS5-knockout-mic.pdf Accepted version application/pdf 1.18MB 150 Title ADAMTS4 and ADAMTS5 knockout mice are protected from versican but not aggrecan or brevican proteolysis during spinal cord injury Author(s) Demircan, K Topcu, V Takigawa, T Akyol, S Yonezawa, T Ozturk, G Ugurcu, V Hasgul, R Yigitoglu, M R Akyol, O McCulloch, D R Hirohata, S Journal name Biomed Research International Volume number 2014 Season Article number 693746 Start page 1 End page 8 Total pages 8 Publisher Hindawi Place of publication New York, NY Publication date 2014 ISSN 2314-6141 Keyword(s) Science & Technology Life Sciences & Biomedicine Biotechnology & Applied Microbiology Medicine, Research & Experimental Research & Experimental Medicine HUMAN ARTICULAR-CARTILAGE THROMBOSPONDIN MOTIFS EXTRACELLULAR-MATRIX DEFICIENT MICE METALLOPROTEINASE MOUSE DISINTEGRIN CLEAVAGE PROTEOGLYCAN DEGRADATION Summary The chondroitin sulfate proteoglycans (CSPGs) aggrecan, versican, and brevican are large aggregating extracellular matrix molecules that inhibit axonal growth of the mature central nervous system (CNS). ADAMTS proteoglycanases, including ADAMTS4 and ADAMTS5, degrade CSPGs, representing potential targets for ameliorating axonal growth-inhibition by CSPG accumulation after CNS injury. We investigated the proteolysis of CSPGs in mice homozygous for Adamts4 or Adamts5 null alleles after spinal cord injury (SCI). ADAMTS-derived 50-60 kDa aggrecan and 50 kDa brevican fragments were observed in Adamts4-/-, Adamts5-/-, and wt mice but not in the sham-operated group. By contrast Adamts4-/- and Adamts5-/- mice were both protected from versican proteolysis with an ADAMTS-generated 70 kDa versican fragment predominately observed in WT mice. ADAMTS1, ADAMTS9, and ADAMTS15 were detected by Western blot in Adamts4-/- mice' spinal cords after SCI. Immunohistochemistry showed astrocyte accumulation at the injury site. These data indicate that aggrecan and brevican proteolysis is compensated in Adamts4-/- or Adamts5-/- mice by ADAMTS proteoglycanase family members but a threshold of versican proteolysis is sensitive to the loss of a single ADAMTS proteoglycanase during SCI. We show robust ADAMTS activity after SCI and exemplify the requirement for collective proteolysis for effective CSPG clearance during SCI. Language eng DOI 10.1155/2014/693746 Field of Research 119999 Medical and Health Sciences not elsewhere classified Socio Economic Objective 929999 Health not elsewhere classified HERDC Research category C1 Refereed article in a scholarly journal ERA Research output type C Journal article Copyright notice ©2014, Biomed Central Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30067858 Document type: Journal Article Collections: Faculty of Health School of Medicine Open Access Collection Related Links Link Description Connect to Elements publication management system Go to link with your DU access privileges   Connect to published version Go to link with your DU access privileges   Author URL Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Thu, 27 Nov 2014, 13:28:54 EST Mon, 08 Dec 2014, 14:29:11 EST Mon, 08 Dec 2014, 15:11:16 EST Mon, 08 Dec 2014, 15:23:15 EST Thu, 18 Dec 2014, 08:49:37 EST Thu, 18 Dec 2014, 08:55:43 EST Wed, 14 Oct 2015, 14:01:13 EST Wed, 06 Dec 2017, 06:11:25 EST Fri, 07 Sep 2018, 17:21:48 EST Filtered Full Citation counts:   Cited 22 times in TR Web of Science Cited 25 times in Scopus Search Google Scholar Access Statistics: 209 Abstract Views, 160 File Downloads  -  Detailed Statistics Created: Mon, 08 Dec 2014, 14:29:11 EST