Validation of a multiplex reverse transcription and pre-amplification method using TaqMan(®) MicroRNA assays. Le Carré,J, Lamon,S and Léger,B 2014, Validation of a multiplex reverse transcription and pre-amplification method using TaqMan(®) MicroRNA assays., Frontiers in genetics, vol. 5, pp. 413-1, doi: 10.3389/fgene.2014.00413. Attached Files Name Description MIMEType Size Downloads lecarre-validationofa-2014.pdf Published version application/pdf 938.02KB 169 Title Validation of a multiplex reverse transcription and pre-amplification method using TaqMan(®) MicroRNA assays. Author(s) Le Carré,J Lamon,S orcid.org/0000-0002-3271-6551 Léger,B Journal name Frontiers in genetics Volume number 5 Start page 413 End page 1 Total pages 7 Publisher Frontiers Research Foundation Place of publication Lausanne, Switzerland Publication date 2014-11-26 ISSN 1664-8021 Keyword(s) human skeletal muscle miRNA multiplexing plasma qRT-PCR Summary Since the discovery of microRNAs (miRNAs), different approaches have been developed to label, amplify and quantify miRNAs. The TaqMan(®) technology, provided by Applied Biosystems (ABIs), uses a stem-loop reverse transcription primer system to reverse transcribe the RNA and amplify the cDNA. This method is widely used to identify global differences between the expression of 100s of miRNAs across comparative samples. This technique also allows the quantification of the expression of targeted miRNAs to validate observations determined by whole-genome screening or to analyze few specific miRNAs on a large number of samples. Here, we describe the validation of a method published by ABIs on their web site allowing to reverse transcribe and pre-amplify multiple miRNAs and snoRNAs simultaneously. The validation of this protocol was performed on human muscle and plasma samples. Fast and cost efficient, this method achieves an easy and convenient way to screen a relatively large number of miRNAs in parallel. Language eng DOI 10.3389/fgene.2014.00413 Field of Research 119999 Medical and Health Sciences not elsewhere classified Socio Economic Objective 929999 Health not elsewhere classified HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2014, Frontiers Research Foundation Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30069042 Document type: Journal Article Collections: Faculty of Health School of Exercise and Nutrition Sciences Institute for Physical Activity and Nutrition Open Access Collection Related Links Link Description Connect to Elements publication management system Go to link with your DU access privileges   Connect to published version Go to link with your DU access privileges   Author URL Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Thu, 15 Jan 2015, 10:38:36 EST Fri, 16 Jan 2015, 04:01:18 EST Sat, 17 Jan 2015, 05:08:26 EST Fri, 30 Jan 2015, 09:44:05 EST Fri, 30 Jan 2015, 09:49:59 EST Fri, 30 Jan 2015, 09:52:43 EST Fri, 30 Jan 2015, 09:58:24 EST Fri, 30 Jan 2015, 10:04:15 EST Fri, 30 Jan 2015, 10:05:40 EST Thu, 05 Mar 2015, 13:51:32 EST Wed, 07 Sep 2016, 08:14:06 EST Wed, 11 Jan 2017, 09:52:59 EST Wed, 06 Dec 2017, 06:12:09 EST Fri, 07 Sep 2018, 17:23:55 EST Filtered Full Citation counts:   Cited 27 times in TR Web of Science Cited 25 times in Scopus Search Google Scholar Access Statistics: 651 Abstract Views, 170 File Downloads  -  Detailed Statistics Created: Thu, 15 Jan 2015, 10:38:36 EST