Studies on the interaction between malanin and metal ions by fluorescence spectra methods
Yuan, Y., Zhang, W., Dai, J-H., Liu, X-F., Wang, H-B., Barrow, Colin J. and Yang, Wenrong 2014, Studies on the interaction between malanin and metal ions by fluorescence spectra methods, Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis, vol. 34, no. 12, pp. 3297-3300, doi: 10.3964/j.issn.1000-0593(2014)12-3297-04.
A novel protein with anti-tumor activities named malanin was isolated and purified from an endemic plant in Yunnan and Guangxi provinces. Effects of copper ion, silver ion and calcium ion on malanin and apo-malanin fluorescence spectra were studied. The results showed that copper ion leads to obvious statistic quenching of malanin and apo-malanin fluorescence. The dissociation constant of them from malanin and apo-malanin were about 2.37×10-4 and 2.66×10-4 mol·L-1, respectively. The silver ion did not have quenching action on malanin fluorescence, but it had statistic quenching effect on apo-malanin fluorescence, and its dissociation constant was 2.37×10-4 mol·L-1. Calcium ion did not have quenching action on malanin and apo-malanin fluorescence. It plays an important role in keeping malanin natural conformation.
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