Hyperaccumulation of zinc by Noccaea caerulescens results in a cascade of stress responses and changes in the elemental profile Foroughi,S, Baker,AJM, Roessner,U, Johnson,AAT, Bacic,A and Callahan,DL 2014, Hyperaccumulation of zinc by Noccaea caerulescens results in a cascade of stress responses and changes in the elemental profile, Metallomics, vol. 6, no. 9, pp. 1671-1682, doi: 10.1039/c4mt00132j. Attached Files Name Description MIMEType Size Downloads Title Hyperaccumulation of zinc by Noccaea caerulescens results in a cascade of stress responses and changes in the elemental profile Author(s) Foroughi,S Baker,AJM Roessner,U Johnson,AAT Bacic,A Callahan,DL orcid.org/0000-0002-6384-8717 Journal name Metallomics Volume number 6 Issue number 9 Start page 1671 End page 1682 Publisher RSC Publications Place of publication Cambridge, England Publication date 2014 ISSN 1756-5901 1756-591X Summary Noccaea caerulescens (J. & C. Presl) F. K. Meyer is a metal hyperaccumulating plant which can accumulate more than 2% zinc (Zn) dry tissue mass in its aerial tissues. At this concentration Zn is toxic to most plants due to inhibition of enzyme function, oxidative damage and mineral deficiencies. In this study the elemental and metabolite profiles of N. caerulescens plants grown in four different Zn concentrations were measured. This revealed broad changes in the metabolite and elemental profiles with the hyperaccumulation of Zn. The Zn treated plants exhibited no typical signs of stress such as chlorosis or reduced biomass, however, a range of metabolic stress responses, such as the modification of galactolipids and the major membrane lipids of plastids, and increases in oxylipins, which are precursors to the signalling molecules jasmonic and abscisic acids, as well as the increased synthesis of glucosinolates, was observed. Increases in particular organic acids and the ubiquitous metal cation chelator nicotianamine were also observed. The small molecule metabolite changes observed, however, did not account for the extreme Zn concentrations in the leaf tissue showing that the increase in nicotianamine production most likely negates Fe deficiency. The elemental analyses also revealed significant changes in other essential micronutrients, in particular, significantly lower Mn concentrations in the high Zn accumulating plants, yet higher Fe concentrations. This comprehensive elemental and metabolite analysis revealed novel metabolite responses to Zn and offers evidence against organic acids as metal-storage ligands in N. caerulescens. © 2014 The Royal Society of Chemistry. Language eng DOI 10.1039/c4mt00132j Field of Research 060705 Plant Physiology Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences HERDC Research category C1 Refereed article in a scholarly journal ERA Research output type C Journal article Copyright notice ©2014, RSC Publications Persistent URL http://hdl.handle.net/10536/DRO/DU:30071353 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Life and Environmental Sciences Centre for Biotechnology, Chemistry and Systems Biology Related Links Link Description Connect to published version Go to link with your DU access privileges   Connect to Elements publication management system Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Wed, 18 Mar 2015, 10:35:49 EST Wed, 18 Mar 2015, 10:36:26 EST Thu, 19 Mar 2015, 04:15:25 EST Thu, 19 Mar 2015, 05:17:15 EST Mon, 23 Mar 2015, 10:58:57 EST Mon, 23 Mar 2015, 11:00:01 EST Filtered Full Citation counts:   Cited 15 times in TR Web of Science Cited 16 times in Scopus Search Google Scholar Access Statistics: 664 Abstract Views, 2 File Downloads  -  Detailed Statistics Created: Wed, 18 Mar 2015, 10:36:26 EST