A simple microfluidic chip design for fundamental bioseparation Chan,AS, Danquah,MK, Agyei,D, Hartley,PG and Zhu,Y 2014, A simple microfluidic chip design for fundamental bioseparation, Journal of analytical methods in chemistry, vol. 2014, pp. 1-6, doi: 10.1155/2014/175457. Attached Files Name Description MIMEType Size Downloads agyei-simplemicrofluidic-2014.pdf Published version application/pdf 1.22MB 152 Title A simple microfluidic chip design for fundamental bioseparation Author(s) Chan,AS Danquah,MK Agyei,D orcid.org/0000-0003-2280-4096 Hartley,PG Zhu,Y Journal name Journal of analytical methods in chemistry Volume number 2014 Start page 1 End page 6 Publisher Hindawi Publishing Corporation Place of publication New York, NY Publication date 2014 ISSN 2090-8865 Summary A microchip pressure-driven liquid chromatographic system with a packed column has been designed and fabricated by using poly(dimethylsiloxane) (PDMS). The liquid chromatographic column was packed with mesoporous silica beads of Ia3d space group. Separation of dyes and biopolymers was carried out to verify the performance of the chip. A mixture of dyes (fluorescein and rhodamine B) and a biopolymer mixture (10 kDa Dextran and 66 kDa BSA) were separated and the fluorescence technique was employed to detect the movement of the molecules. Fluorescein molecule was a nonretained species and rhodamine B was attached onto silica surface when dye mixture in deionized water was injected into the microchannel. The retention times for dextran molecule and BSA molecule in biopolymer separation experiment were 45 s and 120 s, respectively. Retention factor was estimated to be 3.3 for dextran and 10.4 for BSA. The selectivity was 3.2 and resolution was 10.7. Good separation of dyes and biopolymers was achieved and the chip design was verified. Language eng DOI 10.1155/2014/175457 Field of Research 090404 Membrane and Separation Technologies 100302 Bioprocessing, Bioproduction and Bioproducts Socio Economic Objective 869899 Environmentally Sustainable Manufacturing not elsewhere classified HERDC Research category C1.1 Refereed article in a scholarly journal ERA Research output type C Journal article Copyright notice ©2014, Hindawi Publishing Corporation Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30071774 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Life and Environmental Sciences Open Access Collection Related Links Link Description Connect to Elements publication management system Go to link with your DU access privileges   Connect to published version Go to link with your DU access privileges   Author URL Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Thu, 26 Mar 2015, 08:22:25 EST Tue, 07 Apr 2015, 09:00:58 EST Tue, 07 Apr 2015, 09:01:33 EST Wed, 08 Apr 2015, 05:00:08 EST Thu, 09 Apr 2015, 06:10:20 EST Fri, 10 Apr 2015, 13:35:56 EST Fri, 10 Apr 2015, 13:36:39 EST Tue, 14 Apr 2015, 12:29:34 EST Tue, 14 Apr 2015, 12:36:56 EST Tue, 14 Apr 2015, 15:09:38 EST Wed, 07 Sep 2016, 08:49:39 EST Wed, 06 Dec 2017, 06:13:54 EST Fri, 07 Sep 2018, 17:27:57 EST Filtered Full Citation counts:   Cited 0 times in TR Web of Science Cited 15 times in Scopus Search Google Scholar Access Statistics: 136 Abstract Views, 152 File Downloads  -  Detailed Statistics Created: Tue, 07 Apr 2015, 09:01:33 EST