Estimation of accumulated lethality under pressure-assisted thermal processing Nguyen,LT, Balasubramaniam,VM and Ratphitagsanti,W 2014, Estimation of accumulated lethality under pressure-assisted thermal processing, Food and bioprocess technology, vol. 7, no. 3, pp. 633-644, doi: 10.1007/s11947-013-1140-6. Attached Files Name Description MIMEType Size Downloads Title Estimation of accumulated lethality under pressure-assisted thermal processing Author(s) Nguyen,LT Balasubramaniam,VM Ratphitagsanti,W Journal name Food and bioprocess technology Volume number 7 Issue number 3 Start page 633 End page 644 Publisher Springer Place of publication New York, NY Publication date 2014-03 ISSN 1935-5130 1935-5149 Keyword(s) Accumulated lethality Bacterial spores F value Pressure-assisted thermal processing Temperature distribution Summary A study was conducted to develop an integrated process lethality model for pressure-assisted thermal processing (PATP) taking into consideration the lethal contribution of both pressure and heat on spore inactivation. Assuming that the momentary inactivation rate was dependent on the survival ratio and momentary pressure-thermal history, a differential equation was formulated and numerically solved using the Runge-Kutta method. Published data on combined pressure-heat inactivation of Bacillus amyloliquefaciens spores were used to obtain model kinetic parameters that considered both pressure and thermal effects. The model was experimentally validated under several process scenarios using a pilot-scale high-pressure food processor. Using first-order kinetics in the model resulted in the overestimation of log reduction compared to the experimental values. When the n th-order kinetics was used, the computed accumulated lethality and the log reduction values were found to be in reasonable agreement with the experimental data. Within the experimental conditions studied, spatial variation in process temperature resulted up to 3.5 log variation in survivors between the top and bottom of the carrier basket. The predicted log reduction of B. amyloliquefaciens spores in deionized water and carrot purée had satisfactory accuracy (1.07-1.12) and regression coefficients (0.83-0.92). The model was also able to predict log reductions obtained during a double-pulse treatment conducted using a pilot-scale high-pressure processor. The developed model can be a useful tool to examine the effect of combined pressure-thermal treatment on bacterial spore lethality and assess PATP microbial safety. © 2013 Springer Science+Business Media New York. Language eng DOI 10.1007/s11947-013-1140-6 Field of Research 119999 Medical and Health Sciences not elsewhere classified Socio Economic Objective 929999 Health not elsewhere classified HERDC Research category C1.1 Refereed article in a scholarly journal ERA Research output type C Journal article Copyright notice ©2014, Springer Persistent URL http://hdl.handle.net/10536/DRO/DU:30072032 Document type: Journal Article Collections: Faculty of Health School of Medicine Related Links Link Description Connect to published version Go to link with your DU access privileges   Connect to Elements publication management system Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Thu, 02 Apr 2015, 14:26:41 EST Thu, 02 Apr 2015, 14:27:31 EST Fri, 03 Apr 2015, 04:03:02 EST Thu, 09 Apr 2015, 06:10:42 EST Mon, 13 Apr 2015, 12:45:53 EST Mon, 13 Apr 2015, 12:47:07 EST Filtered Full Citation counts:   Cited 14 times in TR Web of Science Cited 17 times in Scopus Search Google Scholar Access Statistics: 225 Abstract Views, 1 File Downloads  -  Detailed Statistics Created: Thu, 02 Apr 2015, 14:27:31 EST