A general method to eliminate laboratory induced recombinants during massive, parallel sequencing of cDNA library. Waugh,C, Cromer,D, Grimm,A, Chopra,A, Mallal,S, Davenport,M and Mak,J 2015, A general method to eliminate laboratory induced recombinants during massive, parallel sequencing of cDNA library., Virology Journal, vol. 12, no. 1, pp. 55, doi: 10.1186/s12985-015-0280-x. Attached Files Name Description MIMEType Size Downloads waugh-ageneralmethod-2015.pdf Published version application/pdf 1.15MB 152 Title A general method to eliminate laboratory induced recombinants during massive, parallel sequencing of cDNA library. Author(s) Waugh,C Cromer,D Grimm,A Chopra,A Mallal,S Davenport,M Mak,J orcid.org/0000-0002-5229-5707 Journal name Virology Journal Volume number 12 Issue number 1 Start page 55 Publisher BioMed Central Place of publication London, Eng. Publication date 2015-04 ISSN 1743-422X Summary Massive, parallel sequencing is a potent tool for dissecting the regulation of biological processes by revealing the dynamics of the cellular RNA profile under different conditions. Similarly, massive, parallel sequencing can be used to reveal the complexity of viral quasispecies that are often found in the RNA virus infected host. However, the production of cDNA libraries for next-generation sequencing (NGS) necessitates the reverse transcription of RNA into cDNA and the amplification of the cDNA template using PCR, which may introduce artefact in the form of phantom nucleic acids species that can bias the composition and interpretation of original RNA profiles. Language eng DOI 10.1186/s12985-015-0280-x Field of Research 110804 Medical Virology Socio Economic Objective 920109 Infectious Diseases HERDC Research category C1 Refereed article in a scholarly journal ERA Research output type C Journal article Grant ID NHMRC Project Grant No 1025270 Copyright notice ©2015, BioMed Central Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30072620 Document type: Journal Article Collections: Faculty of Health School of Medicine Open Access Collection Related Links Link Description Connect to Elements publication management system Go to link with your DU access privileges   Connect to published version Go to link with your DU access privileges   Author URL Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Thu, 23 Apr 2015, 08:57:39 EST Fri, 24 Apr 2015, 05:00:29 EST Tue, 28 Apr 2015, 08:15:18 EST Sat, 09 May 2015, 06:09:28 EST Tue, 12 May 2015, 14:43:02 EST Tue, 12 May 2015, 14:44:04 EST Wed, 13 May 2015, 09:56:54 EST Fri, 15 May 2015, 14:01:02 EST Wed, 27 May 2015, 12:55:59 EST Mon, 12 Oct 2015, 16:04:57 EST Wed, 14 Oct 2015, 14:23:08 EST Wed, 07 Sep 2016, 09:04:52 EST Wed, 06 Dec 2017, 06:14:25 EST Fri, 07 Sep 2018, 17:30:02 EST Filtered Full Citation counts:   Cited 13 times in TR Web of Science Cited 13 times in Scopus Search Google Scholar Access Statistics: 355 Abstract Views, 154 File Downloads  -  Detailed Statistics Created: Thu, 23 Apr 2015, 08:57:39 EST